Multistep liquid-phase lithography for fast prototyping of microfluidic free-flow-electrophoresis chips

Multistep liquid-phase lithography for fast prototyping of microfluidic free-flow-electrophoresis chips
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用于微流控自由流动电泳芯片快速原型制作的多步液相光刻

DOI:
10.1007/s00216-011-5351-2
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发表时间:
2011
影响因子:
4.3
通讯作者:
D. Belder
D. Belder
中科院分区:
化学2区
文献类型:
--
作者:
S. Jezierski;L. Gitlin;S. Nagl;D. Belder

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我们提出了一种快速、通用的方法来制作功能齐全的微自由流电泳片。采用多步骤液相光刻技术,在两个玻片之间产生微流控结构,省去了麻烦的键合步骤或成本密集的母版结构。利用光致可定义聚合物聚乙二醇二甲基丙烯酸酯(聚乙二醇二甲基丙烯酸酯)的一种新的无间隔基方法,产生了高度仅为25μm的亲水通道的微流控器件。微流控芯片的特点是电极通道和分离床之间的离子渗透隔离墙和亲水性表面。该芯片的性能是通过自由流动分离荧光黄原染料和荧光标记的氨基酸证明的。
We present a fast and versatile method to produce functional micro free-flow electrophoresis chips. Microfluidic structures were generated between two glass slides applying multistep liquid-phase lithography, omitting troublesome bonding steps or cost-intensive master structures. Utilizing a novel spacer-less approach with the photodefinable polymer polyethyleneglycol dimethacrylate (PEG-DA), microfluidic devices with hydrophilic channels of only 25 μm in height were generated. The microfluidic chips feature ion-permeable segregation walls between the electrode channels and the separation bed and hydrophilic surfaces. The performance of the chip is demonstrated by free-flow electrophoretic separation of fluorescent xanthene dyes and fluorescently labeled amino acids.
使用无电极注塑芯片进行自由流动电泳
DOI: --
发表时间: 2011
期刊: MOEMS-MEMS
影响因子: --
作者:
Stefan Köhler;H. Becker;V. Beushausen;W. Hüttner;H. Wackerbarth;E. Beckert;S. Howitz;D. Belder
通讯作者: D. Belder
DOI: 10.1021/ac0345190
发表时间: 2003-11-01
影响因子: 7.4
作者:
Zhang, CX;Manz, A
通讯作者: Manz, A
DOI: --
发表时间: 2002
期刊: Electrophoresis
影响因子: 2.9
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DOI: 10.1039/b308476k
发表时间: 2003-01-01
期刊: LAB ON A CHIP
影响因子: 6.1
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Xu, Y;Zhang, CX;Manz, A
通讯作者: Manz, A
DOI: 10.1039/b514731j
发表时间: 2006-03-01
期刊: LAB ON A CHIP
影响因子: 6.1
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通讯作者: Schasfoort, RBM