Engineered Coatings for Titanium Implants To Present Ultralow Doses of BMP-7.
Engineered Coatings for Titanium Implants To Present Ultralow Doses of BMP-7.
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DOI：
10.1021/acsbiomaterials.7b01037
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发表时间：
2018-05-14
影响因子：
5.8
通讯作者：
Salmerón-Sánchez M
中科院分区：
文献类型：
--
作者：
Al-Jarsha M;Moulisová V;Leal-Egaña A;Connell A;Naudi KB;Ayoub AF;Dalby MJ;Salmerón-Sánchez M
为了改善钛种植体的临床效果，正在进行的研究导致了多种方法的实施，以提供成骨生长因子，加速和维持骨整合。在这里，我们展示了人骨形态发生蛋白7 （BMP-7）吸附在涂有聚丙烯酸乙酯（PEA）的钛盘上。我们之前的研究表明，PEA促进纤维连接蛋白组织进入纳米网络，暴露整合素和生长因子结合域，从而在整合素/生长因子受体水平上实现协同相互作用。在这里，钛盘涂有PEA和纤维连接蛋白，然后用ng/mL剂量的BMP-7修饰。利用人间充质干细胞研究细胞对这些功能化微环境的反应。细胞粘附、增殖和矿化以及成骨标志物（骨桥蛋白和骨钙素）的表达表明，在没有PEA涂层的情况下，该系统比钛和BMP-7的组合更能促进间充质细胞的骨分化。本研究为利用BMP-7提高钛种植体的生物活性提供了一种新的策略。
The ongoing research to improve the clinical outcome of titanium implants has resulted in the implemetation of multiple approches to deliver osteogenic growth factors accelerating and sustaining osseointegration. Here we show the presentation of human bone morphogenetic protein 7 (BMP-7) adsorbed to titanium discs coated with poly(ethyl acrylate) (PEA). We have previously shown that PEA promotes fibronectin organization into nanonetworks exposing integrin- and growth-factor-binding domains, allowing a synergistic interaction at the integrin/growth factor receptor level. Here, titanium discs were coated with PEA and fibronectin and then decorated with ng/mL doses of BMP-7. Human mesenchymal stem cells were used to investigate cellular responses on these functionalized microenvironments. Cell adhesion, proliferation, and mineralization, as well as osteogenic markers expression (osteopontin and osteocalcin) revealed the ability of the system to be more potent in osteodifferentiation of the mesenchymal cells than combinations of titanium and BMP-7 in absence of PEA coatings. This work represents a novel strategy to improve the biological activity of titanium implants with BMP-7.
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影响因子：
14
作者：
Moulisová V;Gonzalez-García C;Cantini M;Rodrigo-Navarro A;Weaver J;Costell M;Sabater I Serra R;Dalby MJ;García AJ;Salmerón-Sánchez M
通讯作者：
Salmerón-Sánchez M
影响因子：
2.5
作者：
Hudalla, Gregory A.;Kouris, Nicholas A.;Murphy, William L.
通讯作者：
Murphy, William L.
影响因子：
2.6
作者：
German MJ;Osei-Bempong C;Knuth CA;Deehan DJ;Oldershaw RA
通讯作者：
Oldershaw RA
影响因子：
--
作者：
Berginski ME;Gomez SM
通讯作者：
Gomez SM
影响因子：
2.6
作者：
Anoopkumar-Dukie, S;Carey, JB;Allshire, A
通讯作者：
Allshire, A