Phenazine redox cycling enhances anaerobic survival in Pseudomonas aeruginosa by facilitating generation of ATP and a proton-motive force.
Phenazine redox cycling enhances anaerobic survival in Pseudomonas aeruginosa by facilitating generation of ATP and a proton-motive force.
复制标题
DOI：
10.1111/mmi.12566
复制
发表时间：
2014-04
影响因子：
3.6
通讯作者：
Newman DK
中科院分区：
文献类型：
--
作者：
Glasser NR;Kern SE;Newman DK
虽然许多研究探索了铜绿假单胞菌的生长，但相对较少关注其生存。以前，我们报道了内源性非那嗪支持P. aeruginosa的厌氧生存，但其生存的生理机制尚不清楚。在这里，我们证明了非那嗪氧化还原循环使P. aeruginosa将葡萄糖和丙酮酸氧化成醋酸盐，通过醋酸激酶的活性将醋酸盐和ATP合成偶联来促进生存。通过测量细胞内NAD(H)和ATP浓度，我们发现存活与ATP合成相关，而ATP合成在丙酮酸发酵过程中与氧化还原稳态密切相关，而在精氨酸发酵过程中则与氧化还原稳态无关。我们还表明，在发酵过程中，ATP合成酶复合体需要ATP水解来产生质子动力。总之，我们的研究结果表明，非那嗪通过促进氧化还原稳态和ATP合成来维持质子动力。这项工作证明了一个更普遍的原理，即细胞外氧化还原活性分子，如非那嗪，可以通过促进能量产生来扩大微生物的代谢多样性。
While many studies have explored the growth of Pseudomonas aeruginosa, comparatively few have focused on its survival. Previously, we reported that endogenous phenazines support the anaerobic survival of P. aeruginosa, yet the physiological mechanism underpinning survival was unknown. Here, we demonstrate that phenazine redox cycling enables P. aeruginosa to oxidize glucose and pyruvate into acetate, which promotes survival by coupling acetate and ATP synthesis through the activity of acetate kinase. By measuring intracellular NAD(H) and ATP concentrations, we show that survival is correlated with ATP synthesis, which is tightly coupled to redox homeostasis during pyruvate fermentation but not during arginine fermentation. We also show that ATP hydrolysis is required to generate a proton-motive force using the ATP synthase complex during fermentation. Together, our results suggest that phenazines enable maintenance of the proton-motive force by promoting redox homeostasis and ATP synthesis. This work demonstrates the more general principle that extracellular redox-active molecules, such as phenazines, can broaden the metabolic versatility of microorganisms by facilitating energy generation.
登录
查看更多内容
影响因子：
15.9
作者：
BRITIGAN, BE;ROEDER, TL;COX, CD
通讯作者：
COX, CD
影响因子：
3.2
作者：
Eschbach, M;Schreiber, K;Schobert, M
通讯作者：
Schobert, M
影响因子：
3.2
作者：
Klein, Adam H.;Shulla, Ana;Wolfe, Alan J.
通讯作者：
Wolfe, Alan J.
影响因子：
3.6
作者：
Dietrich, Lars E. P.;Price-Whelan, Alexa;Newman, Dianne K.
通讯作者：
Newman, Dianne K.
DOI：
10.1073/pnas.59.2.484
发表时间：
1968-01-01
影响因子：
11.1
作者：
HOPFER, U;LEHNINGER, AL;THOMPSON, TE
通讯作者：
THOMPSON, TE