Isolation of a human T-lymphotropic virus type I strain from Australian aboriginals
Isolation of a human T-lymphotropic virus type I strain from Australian aboriginals
复制标题
从澳大利亚原住民中分离出 I 型人类 T 淋巴细胞病毒株
作者：
Ivan Bastian;Joy Gardner;Daryl Webb;Ian GARDNERt;Menzies
在从健康血清阳性澳大利亚土著建立的CD 4 + T淋巴细胞培养物中分离到人嗜T淋巴细胞病毒I型（HTLV-I）株。该分离株，鉴定为HTLV-IMSHR-1，通过单克隆抗体的免疫荧光，通过培养上清液中gag编码蛋白p24的存在，以及通过共培养导致来自HTLV-I阴性供体的淋巴细胞的感染和转化来检测。应用聚合酶链反应（PCR）技术，对HTLV-1（MSHR-1）前病毒基因组的env基因、pol区和pX区进行了扩增和序列测定。与原型菌株的包膜序列的比较显示高达7%的分歧在核苷酸水平和3.1至4.3%的预测氨基酸序列的分歧。系统发育分析表明，澳大利亚和美拉尼西亚分离株是相关的。与单克隆抗体的差异反应性表明，HTLV-1（MSHR-1）的gag蛋白p19也是不同的。原型HTLV-I分离株和澳大利亚-美拉尼西亚变体之间抗原差异的可能性需要进一步研究，因为这将对血清学诊断和疫苗开发产生影响。
A human T-lymphotropic virus type I (HTLV-I) strain was isolated in a CD4+ T-lymphocyte culture established from a healthy seropositive Australian Aboriginal. This isolate, identified as HTLV-IMSHR-1, was detected by immunofluorescence with monoclonal antibodies, by the presence of gag-encoded protein p24 in the culture supernatant, and by cocultivation leading to infection and transformation of lymphocytes from an HTLV-I-negative donor. By using the polymerase chain reaction technique, the env gene and segments of the pol and pX regions of the proviral genome of HTLV-I(MSHR-1) were amplified and sequenced. Comparison with the envelope sequences of prototype strains revealed up to 7% divergence at the nucleotide level and 3.1 to 4.3% divergence in the predicted amino acid sequence. Phylogenetic analysis showed that the Australian and Melanesian isolates are related. Differential reactivity with monoclonal antibodies suggests that gag protein p19 of HTLV-I(MSHR-1) is also divergent. The potential for antigenic divergence between the prototype HTLV-I isolates and the Austro-Melanesian variants requires further investigation, because it would have implications for serodiagnosis and vaccine development.
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来自北美和非洲患者的人类 T 淋巴细胞病毒 1 型分离株的序列变异有限。
影响因子：
3.7
作者：
Paine,E;Garcia,J;Philpott,TC;Shaw,G;Ratner,L
通讯作者：
Ratner,L
影响因子：
56.9
作者：
CIANCIOLO, GJ;COPELAND, TD;SNYDERMAN, R
通讯作者：
SNYDERMAN, R
影响因子：
1.5
作者：
GALLAHER, WR;BALL, JM;MONTELARO, RC
通讯作者：
MONTELARO, RC
使用 env 编码的合成肽和 gp46 单克隆抗体绘制人 T 细胞白血病病毒 I 型 (HTLV-I) gp46 和 gp21 包膜糖蛋白的免疫原性区域。
DOI：
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发表时间：
1989
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Palker,TJ;Tanner,ME;Scearce,RM;Streilein,RD;Clark,ME;Haynes,BF
通讯作者：
Haynes,BF