A proximity-based programmable DNA nanoscale assembly line.
A proximity-based programmable DNA nanoscale assembly line.
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作者：
我们合成具有所需形状和组成的纳米级颗粒的能力提供了通过以受控方式将颗粒组合成更大结构来产生新功能材料和器件的令人兴奋的前景。自组装可以有效地实现这一任务，但可能受到热力学和动力学的限制：反应物，中间体和产物可能在整个组装时间过程中相互碰撞，产生非目标物质而不是目标物质。纳米级组装的另一种方法是使用包含信息的分子（如DNA）来控制相互作用，从而最大限度地减少不同组件之间的不必要串扰。原则上，这种方法应该允许通过固定单独选择的纳米级组件来逐步和程序化地构建目标产品-就像在装配线上制造汽车一样。在这里，我们证明了纳米级装配线确实可以通过三个已知的基于DNA的模块的明智组合来实现：DNA折纸瓦片，其为组装过程提供框架和轨道，包含三个不同的双态DNA机器的盒，所述盒用作可编程的货物捐赠装置并串联连接到瓦片，以及一个DNA步行者，它可以在轨道上从一个设备移动到另一个设备并收集货物。当步行者穿过由折纸瓦片轨道规定的路径时，它顺序地遇到三个DNA装置，这三个DNA装置可以在允许其货物被转移到步行者的“开”状态和不发生转移的“关”状态之间独立地切换。我们使用三种不同类型的金纳米粒子作为货物，并表明实验系统确实允许控制制造八种不同的产品，这些产品可以用三个双态设备获得。
Our ability to synthesize nanometer-scale particles with desired shapes and compositions offers the exciting prospect of generating new functional materials and devices by combining the particles in a controlled fashion into larger structures. Self-assembly can achieve this task efficiently, but may be subject to thermodynamic and kinetic limitations: Reactants, intermediates and products may collide with each other throughout the assembly timecourse to produce non-target instead of target species. An alternative approach to nanoscale assembly uses information-containing molecules such as DNA to control interactions and thereby minimize unwanted crosstalk between different components. In principle, this method should allow the stepwise and programmed construction of target products by fastening individually selected nanoscale components – much as an automobile is built on an assembly line. Here, we demonstrate that a nanoscale assembly line can indeed be realized by the judicious combination of three known DNA-based modules: a DNA origami tile that provides a framework and track for the assembly process, cassettes containing three distinct two-state DNA machines that serve as programmable cargo-donating devices and are attached in series to the tile, and a DNA walker that can move on the track from device to device and collect cargo. As the walker traverses the pathway prescribed by the origami tile track, it encounters sequentially the three DNA devices that can be independently switched between an ‘ON’ state allowing its cargo to be transferred to the walker, and an ‘OFF’ state where no transfer occurs. We use three different types of gold nanoparticles as cargo and show that the experimental system does indeed allow the controlled fabrication of the eight different products that can be obtained with three two-state devices.
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影响因子：
38.3
作者：
通讯作者：
--
影响因子：
15
作者：
Liu, D;Wang, MS;Mao, CD
通讯作者：
Mao, CD
影响因子：
56.9
作者：
CARUTHERS, MH
通讯作者：
CARUTHERS, MH
影响因子：
13.3
作者：
Bath, Jonathan;Green, Simon J.;Turberfield, Andrew J.
通讯作者：
Turberfield, Andrew J.
DOI：
10.1126/science.1170336
发表时间：
2009-04-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Omabegho T;Sha R;Seeman NC
通讯作者：
Seeman NC