The Role of DNA Topoisomerase Binding Protein 1 (TopBP1) in Genome Stability in Arabidopsis.
The Role of DNA Topoisomerase Binding Protein 1 (TopBP1) in Genome Stability in Arabidopsis.
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DNA拓扑异构酶结合蛋白1（TOPBP1）在拟南芥中基因组稳定性中的作用。
DOI：
10.3390/plants10122568
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发表时间：
2021-11-24
期刊：
影响因子：
--
通讯作者：
Sanchez-Moran E
中科院分区：
文献类型：
--
作者：
Parra-Nunez P;Cooper C;Sanchez-Moran E
DNA拓扑异构酶Ⅱ（DNA topoisomerase Ⅱ，TOPII）在DNA拓扑结构中起着重要作用，在高等真核生物的DNA复制、转录、修复、染色体浓缩等生物学过程中也起着重要作用。已经发现TOPII直接与称为拓扑异构酶II结合蛋白1（TopBP 1）的蛋白质相互作用，该蛋白质似乎在DNA复制和修复中也具有重要作用。在这项研究中，我们进行了不同的实验，以评估TopBP1在DNA修复，有丝分裂和减数分裂中的作用，探索TOPII活性和TopBP1之间的关系。我们发现，topbp1突变体拟南芥幼苗对顺铂治疗和抑制TOPII与依托泊苷产生类似的超敏反应水平。此外，我们认识到，有没有显着差异的WT和topbp1幼苗与顺铂和依托泊苷一起处理，这表明在topbp1突变体中的顺铂的超敏反应可能与TOPII和TopBP1之间的功能相互作用。体细胞和减数分裂后期桥出现在topbp1突变体中的频率相似，当TOPII被抑制甲基巴龙，依托泊苷，或ICFR-187。抑制TOPII对减数分裂的影响发生在S期/G2期，表明在减数分裂开始时就可以产生索烃。因此，如果索烃的加工受损，则可以形成一些后期桥。并对第一次分裂和第二次分裂后期桥的出现进行了讨论。
DNA topoisomerase II (TOPII) plays a very important role in DNA topology and in different biological processes such as DNA replication, transcription, repair, and chromosome condensation in higher eukaryotes. TOPII has been found to interact directly with a protein called topoisomerase II binding protein 1 (TopBP1) which also seems to have important roles in DNA replication and repair. In this study, we conducted different experiments to assess the roles of TopBP1 in DNA repair, mitosis, and meiosis, exploring the relationship between TOPII activity and TopBP1. We found that topbp1 mutant seedlings of Arabidopsis thaliana were hypersensitive to cisplatin treatment and the inhibition of TOPII with etoposide produced similar hypersensitivity levels. Furthermore, we recognised that there were no significant differences between the WT and topbp1 seedlings treated with cisplatin and etoposide together, suggesting that the hypersensitivity to cisplatin in the topbp1 mutant could be related to the functional interaction between TOPII and TopBP1. Somatic and meiotic anaphase bridges appeared in the topbp1 mutant at similar frequencies to those when TOPII was inhibited with merbarone, etoposide, or ICFR-187. The effects on meiosis of TOPII inhibition were produced at S phase/G2 stage, suggesting that catenanes could be produced at the onset of meiosis. Thus, if the processing of the catenanes is impaired, some anaphase bridges can be formed. Also, the appearance of anaphase bridges at first and second division is discussed.
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DOI：
10.1083/jcb.201803019
发表时间：
2018-12-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Martinez-Garcia M;Schubert V;Osman K;Darbyshire A;Sanchez-Moran E;Franklin FCH
通讯作者：
Franklin FCH
DOI：
10.1038/nrc2607
发表时间：
2009-05
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
影响因子：
2.6
作者：
Moran, ES;Armstrong, SJ;Jones, GH
通讯作者：
Jones, GH
影响因子：
5.3
作者：
Liu, Kang;Graves, Joshua D.;Lin, Weei-Chin
通讯作者：
Lin, Weei-Chin
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR