TOPII and chromosome movement help remove interlocks between entangled chromosomes during meiosis.
TOPII and chromosome movement help remove interlocks between entangled chromosomes during meiosis.
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DOI：
10.1083/jcb.201803019
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发表时间：
2018-12-03
期刊：
影响因子：
--
通讯作者：
Franklin FCH
中科院分区：
文献类型：
--
作者：
Martinez-Garcia M;Schubert V;Osman K;Darbyshire A;Sanchez-Moran E;Franklin FCH
在减数分裂过程中，不相关的染色体经常联锁，为了完成突触和正常的染色体分离，必须去除这些结构。Martinez-Garcia等人表明，主动去除互锁需要拓扑异构酶II和染色体运动。在减数分裂的合子期，染色体突触的正常进展和同源重组经常导致纠缠染色体之间形成结构互锁。联锁的持续存在直到第一次减数分裂会危及正常的突触和偶尔的染色体分离。然而，它们通常被粗线石去除。据推测，连锁去除需要一个或多个活性过程，可能涉及拓扑异构酶II （TOPII）和/或染色体运动。然而，缺乏实验证据。利用免疫细胞化学和结构照明显微镜（SIM）分析拟南芥的一个半形topII突变体和减数分裂特异性topII RNAi下调，现在使我们能够证明topII在联锁分辨率中的作用。此外，利用影响染色体运动的核孔蛋白nup136突变体进行的分析表明，尽管某些连锁结构的去除需要TOPII活性，但对于其他结构，染色体运动也是必要的。因此，我们的研究表明，至少需要两种机制来确保连锁去除。
During meiosis, unrelated chromosomes frequently become interlocked, and these structures must be removed for complete synapsis and normal chromosome segregation. Martinez-Garcia et al. show that the active removal of interlocks requires topoisomerase II and chromosome movement. During the zygotene stage of meiosis, normal progression of chromosome synapsis and homologous recombination frequently lead to the formation of structural interlocks between entangled chromosomes. The persistence of interlocks through to the first meiotic division can jeopardize normal synapsis and occasionally chromosome segregation. However, they are generally removed by pachytene. It has been postulated that interlock removal requires one or more active processes, possibly involving topoisomerase II (TOPII) and/or chromosome movement. However, experimental evidence has been lacking. Analysis of a hypomorphic topII mutant and a meiosis-specific topII RNAi knockdown of Arabidopsis thaliana using immunocytochemistry and structured illumination microscopy (SIM) has now enabled us to demonstrate a role for TOPII in interlock resolution. Furthermore, analysis using a nucleoporin nup136 mutant, which affects chromosome movement, reveals that although TOPII activity is required for the removal of some interlock structures, for others, chromosome movement is also necessary. Thus, our study demonstrates that at least two mechanisms are required to ensure interlock removal.
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Rap1和拓扑异构酶II在酵母的核和减数分裂染色体中的定位。
影响因子：
7.8
作者：
Klein, F;Laroche, T;Cardenas, M E;Hofmann, J F;Schweizer, D;Gasser, S M
通讯作者：
Gasser, S M
DOI：
10.1007/bf02906519
发表时间：
1981-01-01
期刊：
CARLSBERG RESEARCH COMMUNICATIONS
影响因子：
--
作者：
HOLM, PB;RASMUSSEN, SW;SAGE, J
通讯作者：
SAGE, J
DOI：
10.1007/978-1-60761-103-5_9
发表时间：
2009-01-01
期刊：
MEIOSIS, VOL 2: CYTOLOGICAL METHODS
影响因子：
--
作者：
Armstrong, Susan J.;Sanchez-Moran, Eugenio;Franklin, F. Chris H.
通讯作者：
Franklin, F. Chris H.
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
11.8
作者：
Link J;Paouneskou D;Velkova M;Daryabeigi A;Laos T;Labella S;Barroso C;Pacheco Piñol S;Montoya A;Kramer H;Woglar A;Baudrimont A;Markert SM;Stigloher C;Martinez-Perez E;Dammermann A;Alsheimer M;Zetka M;Jantsch V
通讯作者：
Jantsch V