Genome-Wide Association Study Reveals Both Overlapping and Independent Genetic Loci to Control Seed Weight and Silique Length in Brassica napus.
Genome-Wide Association Study Reveals Both Overlapping and Independent Genetic Loci to Control Seed Weight and Silique Length in Brassica napus.
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全基因组关联研究揭示了控制甘蓝型油菜种子重量和长角果长度的重叠和独立遗传位点
DOI：
10.3389/fpls.2018.00921
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发表时间：
2018
影响因子：
5.6
通讯作者：
Qian W
中科院分区：
文献类型：
--
作者：
Dong H;Tan C;Li Y;He Y;Wei S;Cui Y;Chen Y;Wei D;Fu Y;He Y;Wan H;Liu Z;Xiong Q;Lu K;Li J;Qian W
甘蓝型油菜种子重（SW）是决定种子产量的三个因素之一，籽粒重与硅油长度（SL）呈正相关。然而，种子重（SW）和硅长（SL）之间关系的遗传机制目前尚不清楚。利用全基因组重测序技术，对一个由157个油菜自交系组成的自然群体进行了基因分型研究。全基因组关联研究在A01、A04、A09、C02和C06染色体上发现20个与SW显著相关的snp，单个位点的表型变异解释范围为11.85% ~ 34.58%，平均为25.43%。同时，在A02、A03、A04、A07、A08、A09、C01、C03、C04、C06、C07、C08染色体上检测到742个与SL显著相关的snp，单个位点解释的表型变异范围为4.01 ~ 48.02%，平均为33.33%，其中一半以上的位点在既往研究中未见报道。在A04、A09和C06染色体上，SW和SL都有320个重叠或连锁的snp。结果表明，甘蓝型油菜的SW和SL基因既有重叠位点，也有独立位点。在A09染色体单倍型块上，已知基因BnaA.ARF18的等位基因变异。利用衍生的裂解扩增多态性序列（cleaved amplified polymorphic sequence, dCAPS）标记，在自然种群中鉴定出具有控制作用的SW和SL。这些研究结果对油菜分子育种和油菜分子育种具有重要的指导意义。
Seed weight (SW) is one of three determinants of seed yield, which positively correlates with silique length (SL) in Brassica napus (rapeseed). However, the genetic mechanism underlying the relationship between seed weight (SW) and silique length (SL) is largely unknown at present. A natural population comprising 157 inbred lines in rapeseed was genotyped by whole-genome re-sequencing and investigated for SW and SL over four years. The genome-wide association study identified 20 SNPs in significant association with SW on A01, A04, A09, C02, and C06 chromosomes and the phenotypic variation explained by a single locus ranged from 11.85% to 34.58% with an average of 25.43%. Meanwhile, 742 SNPs significantly associated with SL on A02, A03, A04, A07, A08, A09, C01, C03, C04, C06, C07, and C08 chromosomes were also detected and the phenotypic variation explained by a single locus ranged from 4.01 to 48.02% with an average of 33.33%, out of which, more than half of the loci had not been reported in the previous studies. There were 320 overlapping or linked SNPs for both SW and SL on A04, A09, and C06 chromosomes. It indicated that both overlapping and independent genetic loci controlled both SW and SL in B. napus. On the haplotype block on A09 chromosome, the allele variants of a known gene BnaA.ARF18.a controlling both SW and SL were identified in the natural population by developing derived cleaved amplified polymorphic sequence (dCAPS) markers. These findings are valuable for understanding the genetic mechanism of SW and SL and also for rapeseed molecular breeding programs.
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影响因子：
3.7
作者：
Geng X;Jiang C;Yang J;Wang L;Wu X;Wei W
通讯作者：
Wei W
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
3.3
作者：
Shi, Jiaqin;Li, Ruiyuan;Meng, Jinling
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影响因子：
5.3
作者：
Li N;Shi J;Wang X;Liu G;Wang H
通讯作者：
Wang H
通过拟南芥和芸苔属植物比较作图鉴定甘蓝型油菜种子重量 QTL 候选基因
影响因子：
2.9
作者：
Cai G;Yang Q;Yang Q;Zhao Z;Chen H;Wu J;Fan C;Zhou Y
通讯作者：
Zhou Y