Bioconjugated pluronic triblock-copolymer micelle-encapsulated quantum dots for targeted imaging of cancer: in vitro and in vivo studies.
Bioconjugated pluronic triblock-copolymer micelle-encapsulated quantum dots for targeted imaging of cancer: in vitro and in vivo studies.
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DOI：
10.7150/thno.3456
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发表时间：
2012
期刊：
影响因子：
12.4
通讯作者：
Prasad PN
中科院分区：
文献类型：
--
作者：
Liu L;Yong KT;Roy I;Law WC;Ye L;Liu J;Liu J;Kumar R;Zhang X;Prasad PN
在本研究的早期，CdTe/ZnS核/壳量子点（QDs）被封装在羧化Pluronic F127三嵌段聚合物胶束中，以保持QDs在生物流体中的光学和胶体稳定性。然后，通过利用肿瘤细胞上过表达的FA受体（法尔斯），将叶酸（FA）缀合至QD的表面，用于将QD制剂靶向递送至肿瘤部位。细胞毒性研究表明，QD制剂的体外毒性可忽略不计。体外研究表明，生物缀合的胶束封装的量子点，而不是未缀合的量子点，能够有效地标记Panc-1癌细胞。体内成像研究表明，生物缀合的量子点能够在荷瘤小鼠静脉注射制剂后靶向肿瘤部位。
Early in this study, CdTe/ZnS core/shell quantum dots (QDs) were encapsulated in carboxylated Pluronic F127 triblock polymeric micelle, to preserve the optical and colloidal stability of QDs in biological fluids. Folic acid (FA) was then conjugated to the surface of QDs for the targeted delivery of the QD formulation to the tumor site, by exploiting the overexpressed FA receptors (FARs) on the tumor cells. Cytotoxicity study demonstrated that the QD formulation has negligible in vitro toxicity. The in vitro study showed that the bioconjugated micelle-encapsulated QDs, but not the unconjugated QDs, were able to efficiently label Panc-1 cancer cells. In vivo imaging study showed that bioconjugated QDs were able to target tumor site after intravenous injection of the formulation in tumor-bearing mice.
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影响因子：
12.4
作者：
Huang P;Xu C;Lin J;Wang C;Wang X;Zhang C;Zhou X;Guo S;Cui D
通讯作者：
Cui D
影响因子：
13.3
作者：
Law, Wing-Cheung;Yong, Ken-Tye;Prasad, Paras N.
通讯作者：
Prasad, Paras N.
影响因子：
9.7
作者：
Geszke, Malgorzata;Murias, Marek;Schneider, Raphael
通讯作者：
Schneider, Raphael
影响因子：
19
作者：
Hsu, Yu-Chuan;Chang, Yung-Hsun;Yang, Chia-Min
通讯作者：
Yang, Chia-Min
影响因子：
15
作者：
Bharali, DJ;Lucey, DW;Prasad, PN
通讯作者：
Prasad, PN