Dysfunction of low-density neutrophils in peripheral circulation in patients with sepsis.
Dysfunction of low-density neutrophils in peripheral circulation in patients with sepsis.
复制标题
脓毒症患者外周循环低密度中性粒细胞功能障碍。
DOI：
10.1038/s41598-021-04682-x
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发表时间：
2022-01-13
影响因子：
4.6
通讯作者：
Sun B
中科院分区：
文献类型：
--
作者：
Sun R;Huang J;Yang Y;Liu L;Shao Y;Li L;Sun B
Low-density neutrophils (LDNs) have been described in tumors and various autoimmune diseases, where they exhibit immune dysfunction and alter disease progression. Nevertheless, LDNs have been rarely reported in sepsis. We studied sepsis patients admitted to the intensive care unit. Wright-Giemsa stain assay and Transmission electron microscopy were performed to detect the morphology of neutrophils. Flow cytometry was used to analyze the number and function of LDNs. Concentration of cytokines was measured using ELISA. Neutrophil chemotaxis was examined using an under-agarose chemotaxis model. We found that LDNs were significantly elevated in patients with sepsis. Phenotypes and morphological characteristics suggest that LDNs may be formed by mixtures of neutrophils at various maturation stages. In vitro experiments showed that LDN formation was closely associated with neutrophil degranulation. We preliminarily discussed changes in immune function in LDNs. Compared with high-density neutrophils, expression levels of CXC chemokine receptor 4 on LDN surfaces were increased, phagocytotic capacity was decreased, and life span was prolonged. The chemotactic ability of LDNs was significantly reduced, possibly related to the increased expression of P2X1. These data suggest that LDNs are essential components of neutrophils in sepsis. To clarify the source and dysfunction mechanism of LDN in sepsis may be helpful for the diagnosis and treatment of sepsis in the future.
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影响因子：
3.7
作者：
Li, Yajiao;Li, Huiyu;Jie, Shenghua
通讯作者：
Jie, Shenghua
DOI：
10.4049/jimmunol.1100123
发表时间：
2011-07-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lin AM;Rubin CJ;Khandpur R;Wang JY;Riblett M;Yalavarthi S;Villanueva EC;Shah P;Kaplan MJ;Bruce AT
通讯作者：
Bruce AT
影响因子：
4
作者：
Rosales C
通讯作者：
Rosales C
影响因子：
--
作者：
HACBARTH, E;KAJDACSYBALLA, A
通讯作者：
KAJDACSYBALLA, A
影响因子：
6.7
作者：
Bowers NL;Helton ES;Huijbregts RP;Goepfert PA;Heath SL;Hel Z
通讯作者：
Hel Z