Faecal and mucosal microbiota in patients with functional gastrointestinal disorders: Correlation with toll-like receptor 2/toll-like receptor 4 expression.
Faecal and mucosal microbiota in patients with functional gastrointestinal disorders: Correlation with toll-like receptor 2/toll-like receptor 4 expression.
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DOI：
10.3748/wjg.v23.i36.6665
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发表时间：
2017-09-28
影响因子：
4.3
通讯作者：
Han Y
中科院分区：
文献类型：
--
作者：
Dong LN;Wang JP;Liu P;Yang YF;Feng J;Han Y
旨在调查中国功能性胃肠病 (FGID) 患者的肠腔微生物群 (LM) 和粘膜相关微生物群 (MAM)，并检查这些群落与 Toll 样受体 (TLR) 2 和 TLR4 表达之间的关联。经胃肠病学家证实患有 FGID 症状的 32 名中国受试者参加了这项研究。在柔性结肠镜检查之前（粪便）和期间（粘膜）从受试者收集新鲜粪便样本和降结肠粘膜活检。为了对样本进行分析，我们对 16S rRNA 基因的 V3-V4 区域进行了高通量测序，并进行了逆转录 (RT)-PCR 来检测结肠 TLR2 和 TLR4 的表达。检查了粪便和粘膜微生物群的差异，并进行了相关网络分析。粪便样本的微生物群明显比粘膜样本的微生物群更加多样化和丰富，并且 LM 和 MAM 种群存在显着差异。 TLR2表达量与TLR4表达量呈显着正相关。在 MAM 样本中，属于瘤胃球菌科的粪杆菌属和瘤胃球菌属与 TLR4 表达呈负相关（分别为 r = -0.45817，P = 0.0083 和 r = -0.5306，P = 0.0018）。肉杆菌科的颗粒菌和链球菌科的链球菌与 TLR2 表达呈负相关（分别为 r = -0.5573，P = 0.0010 和 r = -0.5435，P = 0.0013）。在 LM 样本中，门、纲或目水平的检查显示与 TLR4 表达没有相关性。瘤胃球菌科的粪杆菌和链球菌科的链球菌与 TLR2 表达呈负相关（分别为 r = -0.5743，P = 0.0058 和 r = -0.3905，P = 0.0271）。中国 FGID 患者的 LM 和 MAM 微生物组成不同。 TLR 的表达可能受到肠道细菌类型的影响。
To investigate the intestinal luminal microbiota (LM) and mucosa-associated microbiota (MAM) in Chinese patients with functional gastrointestinal disorders (FGIDs) and examine the association between these communities and the expression of toll-like receptor (TLR) 2 and TLR4. Thirty-two Chinese subjects who suffered from symptoms of FGIDs, as confirmed by gastroenterologists, were enrolled in this study. Fresh faecal samples and descending colonic mucosal biopsies were collected from the subjects before (faecal) and during (mucosal) flexible colonoscopy. For analysis of the samples, we performed high-throughput sequencing of the V3-V4 region of the 16S rRNA gene and reverse transcription (RT)-PCR to detect the expression of colonic TLR2 and TLR4. Differences in the stool and mucosal microbiota were examined and a correlation network analysis was performed. The microbiota of faecal samples was significantly more diverse and richer than that of the mucosal samples, and the LM and MAM populations differed significantly. TLR2 expression showed a significant positive correlation with TLR4 expression. In the MAM samples, the genera Faecalibacterium and Ruminococcus, which belong to the family Ruminococcaceae, were inversely correlated with TLR4 expression (r = -0.45817, P = 0.0083 and r = -0.5306, P = 0.0018, respectively). Granulicatella, which belongs to Carnobacteriaceae, and Streptococcus, which belongs to Streptococcaceae, were inversely correlated with TLR2 expression (r = -0.5573, P = 0.0010 and r = -0.5435, P = 0.0013, respectively). In the LM samples, examination at phylum, class, or order level revealed no correlation with TLR4 expression. Faecalibacterium, which belongs to Ruminococcaceae, and Streptococcus, which belongs to Streptococcaceae, were inversely correlated with TLR2 expression (r = -0.5743, P = 0.0058 and r = -0.3905, P = 0.0271, respectively). Microbial compositions of LM and MAM in Chinese patients with FGIDs are different. Expression of TLRs may be affected by the type of bacteria that are present in the gut.
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影响因子：
4.1
作者：
Frosali S;Pagliari D;Gambassi G;Landolfi R;Pandolfi F;Cianci R
通讯作者：
Cianci R
高通量测序揭示了健康人粘膜和管腔壁龛内不同的微生物种群
影响因子：
12.2
作者：
Ringel, Yehuda;Maharshak, Nitsan;Carroll, Ian M.
通讯作者：
Carroll, Ian M.
影响因子：
5.7
作者：
Ekmekciu I;von Klitzing E;Fiebiger U;Neumann C;Bacher P;Scheffold A;Bereswill S;Heimesaat MM
通讯作者：
Heimesaat MM
DOI：
10.1042/bcj20160510
发表时间：
2017-05-16
期刊：
The Biochemical journal
影响因子：
--
作者：
Thursby E;Juge N
通讯作者：
Juge N
影响因子：
24.5
作者：
Machiels, Kathleen;Joossens, Marie;Vermeire, Severine
通讯作者：
Vermeire, Severine