GpsB Coordinates Cell Division and Cell Surface Decoration by Wall Teichoic Acids in Staphylococcus aureus.
GpsB Coordinates Cell Division and Cell Surface Decoration by Wall Teichoic Acids in Staphylococcus aureus.
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DOI：
10.1128/spectrum.01413-22
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发表时间：
2022-06-29
影响因子：
3.7
通讯作者：
Eswara, Prahathees J.
中科院分区：
文献类型：
--
作者：
Hammond, Lauren R.;Sacco, Michael D.;Khan, Sebastian J.;Spanoudis, Catherine;Hough-Neidig, Abigail;Chen, Yu;Eswara, Prahathees J.
细菌细胞分裂是一个复杂且高度调控的过程，需要许多不同蛋白质的协调。尽管在模式生物中做了大量的工作，但我们对非典型生物（包括重要的人类病原体）中调节细胞分裂的系统的理解还远远没有完成。一种这样的生物是金黄色葡萄球菌，一种缺乏已知细胞分裂调节蛋白的球形细菌。GpsB是厚壁菌门中的一种保守蛋白，近年来的研究为厚壁菌门中细胞分裂调控提供了新的视角。金黄色葡萄球菌和其他相关生物。已经揭示，GpsB在多个物种中协调细胞分裂和细胞壁合成。In S.金黄色葡萄球菌，我们以前已经表明，GpsB直接调节FtsZ聚合。在这项研究中，使用枯草芽孢杆菌作为一种工具，我们分离的自发抑制剂，废除了S。金黄色葡萄球菌Gps B在B中过量产生。枯草芽孢杆菌通过表征，我们鉴定了几个对GpsB功能重要的残基。此外，我们还发现GpsB在S.金黄色。具体而言，我们表明，GpsB直接与WTA出口蛋白TarG相互作用。我们还确定了GpsB中对这种相互作用至关重要的区域。S. aureus认为WTA机制是分裂复合体的一部分。综上所述，本研究阐明了GpsB在S.金黄色葡萄球菌通过直接连接细胞分裂和WTA介导的细胞表面装饰的严格调节的细胞周期过程。重要性细菌的胞质分裂涉及到几个关键的细胞分裂蛋白和其他参与建立一个强大的细胞包膜的因素的复杂编排。磷壁酸的存在是革兰氏阳性细胞壁的特征。通过表征金黄色葡萄球菌GpsB的作用，在这种生物体中的一种必需的细胞分裂蛋白，我们已经发现了GpsB在壁磷壁酸（WTA）生物合成中的额外作用。我们表明，GpsB直接与WTA出口复杂的TarG相互作用。我们还表明，这种功能的GpsB可能是保守的其他GpsB同系物的GpsB和WTA出口复杂遵循类似的本地化模式。已经表明WTA作为分子信号来控制自溶酶的活性，特别是在分离连体子细胞期间。因此，我们的研究结果表明，GpsB，除了在细胞分裂中发挥作用，也可能有助于协调WTA生物合成。
Bacterial cell division is a complex and highly regulated process requiring the coordination of many different proteins. Despite substantial work in model organisms, our understanding of the systems regulating cell division in noncanonical organisms, including critical human pathogens, is far from complete. One such organism is Staphylococcus aureus, a spherical bacterium that lacks known cell division regulatory proteins. Recent studies on GpsB, a protein conserved within the Firmicutes phylum, have provided insight into cell division regulation in S. aureus and other related organisms. It has been revealed that GpsB coordinates cell division and cell wall synthesis in multiple species. In S. aureus, we have previously shown that GpsB directly regulates FtsZ polymerization. In this study, using Bacillus subtilis as a tool, we isolated spontaneous suppressors that abrogate the lethality of S. aureus GpsB overproduction in B. subtilis. Through characterization, we identified several residues important for the function of GpsB. Furthermore, we discovered an additional role for GpsB in wall teichoic acid (WTA) biosynthesis in S. aureus. Specifically, we show that GpsB directly interacts with the WTA export protein TarG. We also identified a region in GpsB that is crucial for this interaction. Analysis of TarG localization in S. aureus suggests that WTA machinery is part of the divisome complex. Taken together, this research illustrates how GpsB performs an essential function in S. aureus by directly linking the tightly regulated cell cycle processes of cell division and WTA-mediated cell surface decoration. IMPORTANCE Cytokinesis in bacteria involves an intricate orchestration of several key cell division proteins and other factors involved in building a robust cell envelope. Presence of teichoic acids is a signature characteristic of the Gram-positive cell wall. By characterizing the role of Staphylococcus aureus GpsB, an essential cell division protein in this organism, we have uncovered an additional role for GpsB in wall teichoic acid (WTA) biosynthesis. We show that GpsB directly interacts with TarG of the WTA export complex. We also show that this function of GpsB may be conserved in other GpsB homologs as GpsB and the WTA exporter complex follow similar localization patterns. It has been suggested that WTA acts as a molecular signal to control the activity of autolytic enzymes, especially during the separation of conjoined daughter cells. Thus, our results reveal that GpsB, in addition to playing a role in cell division, may also help coordinate WTA biogenesis.
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影响因子：
3.6
作者：
Domínguez-Cuevas P;Porcelli I;Daniel RA;Errington J
通讯作者：
Errington J
DOI：
10.1038/nrmicro.2016.26
发表时间：
2016-04
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Haeusser DP;Margolin W
通讯作者：
Margolin W
影响因子：
4.9
作者：
Campbell, Jennifer;Singh, Atul K.;Walker, Suzanne
通讯作者：
Walker, Suzanne
影响因子：
4.8
作者：
Frankel, Matthew B.;Schneewind, Olaf
通讯作者：
Schneewind, Olaf
DOI：
10.1073/pnas.1004304107
发表时间：
2010-11-02
影响因子：
11.1
作者：
Atilano, Magda L.;Pereira, Pedro M.;Filipe, Sergio R.
通讯作者：
Filipe, Sergio R.