The T cell antigen receptor complex expressed on normal peripheral blood CD4-, CD8- T lymphocytes. A CD3-associated disulfide-linked gamma chain heterodimer
The T cell antigen receptor complex expressed on normal peripheral blood CD4-, CD8- T lymphocytes. A CD3-associated disulfide-linked gamma chain heterodimer
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T细胞抗原受体复合物表达于正常外周血CD4-、CD8-T淋巴细胞上。
DOI：
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发表时间：
1987
影响因子：
15.3
通讯作者：
A. Weiss
中科院分区：
文献类型：
--
作者：
L. Lanier;N. Federspiel;J. Ruitenberg;J. Phillips;J. Allison;D. Littman;A. Weiss
从正常外周血T淋巴细胞中建立il -2依赖性细胞系，既不表达CD4也不表达CD8分化抗原。来自15个不同供体的CD3+，4-，8-细胞系与WT31（一种针对T细胞抗原受体α / β异源二聚体的单抗）没有反应。Anti-Leu-4 mAb用于从125i标记的CD3+，4-,8- (WT31-) T细胞中分离CD3/T细胞抗原受体复合物。使用保留CD3/T细胞抗原受体复合物的洗涤剂条件，由大约45-和大约40-（或大约37-）kD亚基组成的大约90 kD二硫连接的异二聚体与不变的20-29-kD CD3复合物共免疫沉淀。通过非平衡pH梯度电泳分析这些成分表明，大约40-kD和大约37-kD亚基相似，与更基本的大约45-kD亚基截然不同。这三个亚基都不能与针对β链框架表位的抗体发生反应。针对T细胞受体γ链肽的异源抗血清可与约37- kd和约40-kD的cd3相关蛋白特异性反应，但不能与约45-kD亚基反应。CD3+,4-，8-细胞不能转录大量的功能性1.3-kb β或1.6-kb α mRNA，但产生大量的1.6-kb γ mRNA。Southern blot分析显示，这些CD3+、4-、8-细胞系对γ和β基因进行了重排，表明群体是多克隆的。从正常成人外周血中建立的CD3+，4-，8- T细胞系上CD3相关的二硫化物连接异二聚体的表达与先前报道的CD3+/WT31-细胞系上CD3相关的非二硫化物连接异二聚体的表达形成对比，这些细胞系来自胸腺和免疫缺陷疾病患者的外周血。我们认为这种差异可能是由于在T淋巴细胞中优先使用两种C γ基因。
IL-2-dependent cell lines were established from normal peripheral blood T lymphocytes that express neither CD4 nor CD8 differentiation antigens. CD3+,4-,8- cell lines from 15 different donors failed to react with WT31, an mAb directed against the T cell antigen receptor alpha/beta heterodimer. Anti-Leu-4 mAb was used to isolate the CD3/T cell antigen receptor complex from 125I-labeled CD3+,4-,8- (WT31-) T cells. Using detergent conditions that preserved the CD3/T cell antigen receptor complex, an approximately 90 kD disulfide-linked heterodimer, composed of approximately 45- and approximately 40- (or approximately 37-) kD subunits, was coimmunoprecipitated with the invariant 20-29-kD CD3 complex. Analysis of these components by nonequilibrium pH gradient electrophoresis indicated that the approximately 40-kD and approximately 37-kD subunits were similar, and quite distinct from the more basic approximately 45-kD subunit. None of these three subunits reacted with an antibody directed against a beta chain framework epitope. Heteroantiserum against a T cell receptor gamma chain peptide specifically reacted with both the approximately 37- and approximately 40-kD CD3-associated proteins, but not with the approximately 45-kD subunit. CD3+,4-,8- cells failed to transcribe substantial amounts of functional 1.3-kb beta or 1.6-kb alpha mRNA, but produced abundant 1.6- kb gamma mRNA. Southern blot analysis revealed that these CD3+,4-,8- cell lines rearranged both gamma and beta genes, and indicated that the populations were polyclonal. The expression of a CD3-associated disulfide-linked heterodimer on CD3+,4-,8- T cell lines established from normal, adult peripheral blood contrasts with prior reports describing a CD3-associated non-disulfide-linked heterodimer on CD3+/WT31- cell lines established from thymus and peripheral blood obtained from patients with immunodeficiency diseases. We propose that this discrepancy may be explained by preferential usage of the two C gamma genes in T lymphocytes.
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T 细胞受体基因重排作为 T 细胞肿瘤谱系和克隆性的标记。
DOI：
10.1073/pnas.82.10.3460
发表时间：
1985
影响因子：
11.1
作者：
Flug,F;Pelicci,PG;Bonetti,F;Knowles2nd,DM;Dalla-Favera,R
通讯作者：
Dalla-Favera,R
影响因子：
4.4
作者：
James P. Allison;Bradley W. McIntyre;D. Bloch
通讯作者：
James P. Allison;Bradley W. McIntyre;D. Bloch
单克隆抗体 (WT-31) 的特征，可识别人类 T 细胞抗原受体上的共同表位。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Spits,H;Borst,J;Tax,W;Capel,PJ;Terhorst,C;deVries,JE
通讯作者：
deVries,JE
人类 T 细胞伽马链基因：组织、多样性和重排。
DOI：
10.1126/science.3079918
发表时间：
1986
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Quertermous,T;Murre,C;Dialynas,D;Duby,AD;Strominger,JL;Waldman,TA;Seidman,JG
通讯作者：
Seidman,JG
人 T 淋巴细胞表面 T3 复合物两条重链的表征。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Oettgen,HC;Kappler,J;Tax,WJ;Terhorst,C
通讯作者：
Terhorst,C