Effect of dietary gluten on dendritic cells and innate immune subsets in BALB/c and NOD mice.
Effect of dietary gluten on dendritic cells and innate immune subsets in BALB/c and NOD mice.
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DOI：
10.1371/journal.pone.0118618
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Buschard K
中科院分区：
文献类型：
--
作者：
Larsen J;Weile C;Antvorskov JC;Engkilde K;Nielsen SM;Josefsen K;Buschard K
已知先天免疫系统在对饮食抗原的口服耐受中起重要作用。这在乳糜泻（CD）的发展中很重要，但在1型糖尿病（T1 D）中也可能很重要，并可能解释接受无麸质（GF）饮食的小鼠中T1 D发病率降低的原因。麸质对先天性细胞，特别是树突状细胞（DC）的直接体内作用尚未充分阐明。因此，我们希望研究自发性糖尿病NOD小鼠和健康BALB/c小鼠的先天细胞群，这些小鼠保持在含GF或标准（STD）麸质的饮食中。我们研究了，通过流式细胞术和逆转录-定量聚合酶链反应（qRT-PCR），如果膳食面筋诱导的变化，在激活的DC和选择的先天性细胞在淋巴，胰腺和肠组织中的BALB/c和NOD小鼠的分布。我们发现，GF饮食增加BALB/c脾中的巨噬细胞和BALB/c和NOD脾中的CD 11 c + DC的百分比。严格无麸质（SGF）饮食可增加BALB/c小鼠肠系膜和胰腺淋巴结中CD 103 + DC的百分比，降低CD 11b + DC的百分比。BALB/c小鼠的SGF饮食也降低胰腺淋巴结中CD 40、CCR 7和MHC-II的DC表达。总之，GF饮食改变了BALB/c和NOD小鼠先天免疫系统的组成，并增加了NOD小鼠DC活化标志物的表达。这些结果有助于解释GF NOD小鼠糖尿病发病率低的原因。这种机制可能在1型糖尿病、乳糜泻和非乳糜泻麸质敏感性的发展中很重要。
The innate immune system is known to play an important role in oral tolerance to dietary antigens. This is important in development of celiac disease (CD) but may also be important in type 1 diabetes (T1D), and could potentially explain the reduced incidence of T1D in mice receiving a gluten-free (GF) diet. The direct in vivo effect of gluten on innate cells, and particularly dendritic cells (DC) is not sufficiently clarified. Therefore, we wished to investigate the innate cell populations of spontaneous diabetic NOD mice and healthy BALB/c mice kept on a GF or a standard (STD) gluten containing diet. We studied, by flow cytometry and reverse transcription-quantitative polymerase chain reaction (qRT-PCR), if dietary gluten induces changes in the activation of DCs and distribution of selected innate cells in lymphoid, pancreatic and intestinal tissues in BALB/c and NOD mice. We found that a GF diet increased the percentage of macrophages in BALB/c spleen and of CD11c+ DCs in BALB/c and NOD spleen. Strictly gluten-free (SGF) diet increased the percentage of CD103+ DCs in BALB/c mice and decreased percentages of CD11b+ DCs in mesenteric and pancreatic lymph nodes in BALB/c mice. SGF diet in BALB/c mice also decreased DC expression of CD40, CCR7 and MHC-II in pancreatic lymph nodes. In conclusion, GF diet changes the composition of the innate immune system in BALB/c and NOD mice and increases expression of DC activation markers in NOD mice. These results contribute to the explanation of the low diabetes incidence in GF NOD mice. This mechanism may be important in development of type 1 diabetes, celiac disease and non-celiac gluten sensitivity.
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影响因子：
5.9
作者：
Catassi C;Bai JC;Bonaz B;Bouma G;Calabrò A;Carroccio A;Castillejo G;Ciacci C;Cristofori F;Dolinsek J;Francavilla R;Elli L;Green P;Holtmeier W;Koehler P;Koletzko S;Meinhold C;Sanders D;Schumann M;Schuppan D;Ullrich R;Vécsei A;Volta U;Zevallos V;Sapone A;Fasano A
通讯作者：
Fasano A
DOI：
10.1084/jem.20102660
发表时间：
2012-12-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Junker Y;Zeissig S;Kim SJ;Barisani D;Wieser H;Leffler DA;Zevallos V;Libermann TA;Dillon S;Freitag TL;Kelly CP;Schuppan D
通讯作者：
Schuppan D
影响因子：
3.7
作者：
Beitnes, A. -C. R.;Raki, M.;Jahnsen, F. L.
通讯作者：
Jahnsen, F. L.
影响因子：
7.7
作者：
Creusot RJ;Giannoukakis N;Trucco M;Clare-Salzler MJ;Fathman CG
通讯作者：
Fathman CG
影响因子：
3.7
作者：
Antvorskov JC;Fundova P;Buschard K;Funda DP
通讯作者：
Funda DP