Polymorphisms of human papillomavirus type 16 E7 in Uyghur women with cervical cancer
Polymorphisms of human papillomavirus type 16 E7 in Uyghur women with cervical cancer
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维吾尔族宫颈癌女性人乳头瘤病毒16型E7多态性分析
DOI：
10.31083/j.ejgo.2020.06.5469
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
韩莉莉
中科院分区：
文献类型：
--
作者：
Lin Wang;Sulaiya Husaiyin;Peifeng Xu;Xiaoli Wang;韩莉莉
目的：分析新疆维吾尔族妇女宫颈癌与HPV 16 E7基因突变的关系。方法：选取2008年12月至2010年8月确诊为宫颈癌的维吾尔族妇女80例。收集宫颈组织。从样品中提取DNA。扩增HPV 16 L1用于鉴定感染HPV 16的组织。采用聚合酶链反应（PCR）和DNA测序技术对HPV 16感染组织中HPV 16 E7基因多态性进行分析。结果：60例标本经病理证实为HPV 16感染。在这些样本中，54例成功进行了HPV 16 E7测序，6例观察到E7突变。突变位点为A647 G（2/54）、T760 C（2/54）、G663 A（1/54）、G666 A（1/54）、C790 T（1/54）和T846 C（1/54）。A647 G（2/54）和C790 T（1/54）为错义突变。结论：新疆地区HPV 16 E7基因保守，突变率较低。
Purpose: To analyse the association of HPVl6 E7mutations with cervical cancer among Uyghur women in Xinjiang, China. Methods: From December 2008 to August 2010, 80 Uygur women who were confirmed to have cervical cancer were recruited. Cervical tissues were collected. DNA was extracted from the samples. HPVl6 L1 was amplified for the identification of tissues infected with HPV16. Polymerase chain reactions and DNA sequencing were then performed for polymorphism analysis of HPVl6 E7 in HPV16-infected tissues. Results: A total of 60 samples were confirmed to have HPV16 infection. Among these samples, 54 successfully underwent HPV16 E7 sequencing, and 6 were observed with E7 mutations. The locations of the mutations were A647G (2/54), T760C (2/54), G663A (1/54), G666A (1/54), C790T (1/54), and T846C (1/54). A647G (2/54) and C790T (1/54) were missense mutations. Conclusion: HPV16 E7 is conserved in Xinjiang with a relatively low mutation rate.
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