Cytokine Gene Expression in CD4 Positive Cells of the Japanese Pufferfish, Takifugu rubripes.
Cytokine Gene Expression in CD4 Positive Cells of the Japanese Pufferfish, Takifugu rubripes.
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DOI：
10.1371/journal.pone.0066364
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Korenaga H
中科院分区：
文献类型：
--
作者：
Kono T;Korenaga H
CD 4 + T（Th）细胞是适应性免疫应答的中心组分，并且基于其特异性细胞因子产生模式被分成不同的组。一些报告表明，鱼类具有Th亚群活性类似于哺乳动物。本研究的目的是分离的CD 4 + T细胞从血液中的日本河豚，红鳍东方，并表征其细胞因子的表达谱。我们制备了抗河豚CD 4的特异性抗体，并利用磁性激活细胞分选系统进行了细胞分选。对分选的河豚CD 4+细胞进行形态学特征和细胞标记基因表达分析。CD 4+细胞表达T细胞标记基因，但不表达巨噬细胞或B细胞标记基因。另外，在分选前用脂多糖（LPS）、多聚胞苷酸（polyI：C）、刀豆球蛋白A（ConA）刺激外周血淋巴细胞，然后用多重RT-PCR检测刺激后的CD 4+细胞Th细胞因子的表达。LPS和polyI：C刺激可上调Th 1、Th 17和Treg细胞因子的表达，下调Th 2细胞因子的表达。ConA刺激上调所有Th细胞因子的表达。这些结果表明，鱼类表现出相同的上调Th-特异性细胞因子的表达在哺乳动物中。
CD4+ T (Th) cells are a central component of the adaptive immune response and are divided into distinct sets based on their specific cytokine production pattern. Several reports have suggested that fish possess Th subset activity similar to that of mammals. The aim of the present study was to isolate CD4+ T cells from the blood of Japanese pufferfish, Fugu rubripes, and to characterize their cytokine expression profile. We produced a specific antibody against Fugu CD4 and performed cell sorting with the magnetic activated cell sorting system. Sorted Fugu CD4+ cells were characterized by morphology and expression analysis of cell marker genes. Fugu CD4+ cells expressed T-cell marker genes but not macrophage or B-cell marker genes. In addition, peripheral blood lymphocytes were stimulated with lipopolysaccharide (LPS), polycytidylic acid (polyI:C), concanavalin A (ConA) prior to sorting, and then Multiplex RT-PCR was used to examine the expression of Th cytokines by the stimulated Fugu CD4+ cells. LPS and polyI:C stimulation upregulated the expression of Th1, Th17 and Treg cytokines and downregulated the expression of Th2 cytokines. ConA stimulation upregulated the expression of all Th cytokines. These results suggest that fish exhibit the same upregulation of Th-specific cytokine expression as in mammals.
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DOI：
10.1084/jem.174.3.583
发表时间：
1991-09-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Haanen JB;de Waal Malefijt R;Res PC;Kraakman EM;Ottenhoff TH;de Vries RR;Spits H
通讯作者：
Spits H
影响因子：
64.8
作者：
Guo, Peng;Hirano, Masayuki;Herrin, Brantley R.;Li, Jianxu;Yu, Cuiling;Sadlonova, Andrea;Cooper, Max D.
通讯作者：
Cooper, Max D.
影响因子：
6.5
作者：
Esche, C;Stellato, C;Beck, LA
通讯作者：
Beck, LA
影响因子：
4.4
作者：
Laing, Kerry J.;Zou, Jun J.;Hansen, John D.
通讯作者：
Hansen, John D.
影响因子：
4.7
作者：
Kuo, Chang-Hung;Ko, Ying-Chin;Hung, Chih-Hsing
通讯作者：
Hung, Chih-Hsing