Rhomboid protein 2 of Eimeria maxima provided partial protection against infection by homologous species.
Rhomboid protein 2 of Eimeria maxima provided partial protection against infection by homologous species.
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巨型艾美耳球虫的菱形蛋白 2 提供了针对同源物种感染的部分保护
DOI：
10.1186/s13567-020-00886-7
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发表时间：
2021-02-18
影响因子：
4.4
通讯作者：
Song X
中科院分区：
文献类型：
--
作者：
Chen Y;Tian D;Xu L;Yan R;Li X;Shah MAA;Song X
菱形样蛋白酶（rhomboid -样proteases， rhomboid -样proteases， ROMs）在顶复合体原虫侵袭过程中发挥重要作用，被认为是开发新一代疫苗的候选抗原。在之前的工作中，我们获得了一个最大梅里亚（EmROM2）的ROM2序列。本研究旨在评价EmROM2重组蛋白（rEmROM2）和EmROM2 DNA （pVAX1-EmROM2）对最大耶美meria （e.m.a）感染的免疫原性和保护作用。最大值)。首先采用Western blot法分析rEmROM2的免疫原性。结果表明，rEmROM2能被鸡抗- e识别。maximaserum。逆转录聚合酶链反应（RT-PCR）和Western blot检测显示EmROM2在注射部位有明显的转录和表达。qRT-PCR（实时荧光定量PCR）、流式细胞术和间接ELISA检测结果显示，接种rEmROM2或EmROM2 DNA可显著上调小鼠细胞因子（IFN-γ、IL-2、IL-4、IL-10、IL-17、TGF-β和TNF SF15）的转录水平、CD8+和CD4+T淋巴细胞比例及血清IgG抗体应答。最后，进行了一项疫苗挑战试验，以评估rEmROM2和pVAX1-EmROM2对ste的保护效果。最大值。结果显示，接种rEmROM2或pVAX1-EmROM2可显著减轻肠内病变，减轻体重，减少卵囊输出。抗球虫指数（ACI）在160以上，表明对球虫有部分保护作用。最大值。总之，接种rEmROM2或pVAX1-EmROM2可显著激活体液和细胞介导的免疫，并提供部分预防ste的保护。最大值。这些结果表明EmROM2蛋白和DNA是有希望的ste疫苗候选物。maximainfection。
Rhomboid-like proteases (ROMs) are considered as new candidate antigens for developing new-generation vaccines due to their important role involved in the invasion of apicomplexan protozoa. In prior works, we obtained a ROM2 sequence ofEimeria maxima(EmROM2). This study was conducted to evaluate the immunogenicity and protective efficacy of EmROM2 recombinant protein (rEmROM2) and EmROM2 DNA (pVAX1-EmROM2) against infection byEimeria maxima(E. maxima). Firstly, Western blot assay was conducted to analyze the immunogenicity of rEmROM2. The result showed that rEmROM2 was recognized by chicken anti-E. maximaserum. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot assay revealed apparent transcription and expression of EmROM2 at the injection site. qRT-PCR (quantitative real-time PCR), flow cytometry and indirect ELISA indicated that vaccination with rEmROM2 or EmROM2 DNA significantly upregulated the transcription level of cytokines (IFN-γ, IL-2, IL-4, IL-10, IL-17, TGF-β and TNF SF15), the proportion of CD8+and CD4+T lymphocytes and serum IgG antibody response. Ultimately, a vaccination-challenge trial was performed to evaluate the protective efficacy of rEmROM2 and pVAX1-EmROM2 againstE. maxima. The result revealed that vaccination with rEmROM2 or pVAX1-EmROM2 significantly alleviated enteric lesions, weight loss, and reduced oocyst output caused by challenge infection ofE. maxima, and provided anticoccidial index (ACI) of more than 160, indicating partial protection againstE. maxima. In summary, vaccination with rEmROM2 or pVAX1-EmROM2 activated notable humoral and cell-mediated immunity and provided partial protection againstE. maxima. These results demonstrated that EmROM2 protein and DNA are promising vaccine candidates againstE. maximainfection.
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影响因子：
4
作者：
Lillehoj, HS
通讯作者：
Lillehoj, HS
DOI：
10.1016/j.ijpddr.2014.10.002
发表时间：
2014-12
影响因子：
4
作者：
Chapman, H. David;Jeffers, Thomas K.
通讯作者：
Jeffers, Thomas K.
影响因子：
6.7
作者：
Baker RP;Wijetilaka R;Urban S
通讯作者：
Urban S
影响因子：
9.6
作者：
Dowse, TJ;Soldati, D
通讯作者：
Soldati, D
影响因子：
2.6
作者：
Holdsworth, PA;Conway, DP;Williams, RB
通讯作者：
Williams, RB