Geographical resistome profiling in the honeybee microbiome reveals resistance gene transfer conferred by mobilizable plasmids.
Geographical resistome profiling in the honeybee microbiome reveals resistance gene transfer conferred by mobilizable plasmids.
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蜜蜂微生物组的地理耐药基因组分析揭示了可移动质粒赋予的耐药基因转移。
DOI：
10.1186/s40168-022-01268-1
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发表时间：
2022-05-03
期刊：
影响因子：
15.5
通讯作者：
中科院分区：
文献类型：
--
作者：
抗生素耐药基因（ARG）的传播已成为全球关注的最大环境威胁之一。已发现动物的肠道微生物组是ARG的大型储存库，这也是环境抗生素谱的指标。保守的微生物群使蜜蜂成为一个易于控制和封闭的生态系统，用于研究ARG在肠道细菌中的维持和转移。虽然已经发现蜜蜂肠道细菌具有不同的ARG组，但环境变量的影响以及驱动其分布的机制仍不清楚。我们的特点是两个密切相关的蜜蜂物种，中华蜜蜂和意大利蜜蜂，在中国14个地理位置驯化的肠道resistome。ARG的组成与寄主种类有关，而与地理分布无关。意大利蜜蜂肠道中ARGs含量较高。耐药基因组的地理分布格局适中，几个核心ARG组在A. cerana samples.这些共有的基因主要由蜜蜂特有的肠道成员Gilgellella和Socculgrassella携带。在蜜蜂肠道中经常检测到可转移的ARGs，并且在A.意大利蜜蜂样本。含有链霉素抗性基因簇的蜂肠道共生体的基因组位点与广泛宿主范围的IncQ质粒（环境中的熟练DNA递送系统）的基因组位点几乎相同。通过体外接合实验，我们证实了可移动质粒可以通过接合在蜜蜂肠道共生菌之间转移。此外，在多个地区不同共生体的整合区域中发现了带有片段化基因的“卫星质粒”。我们的研究表明，不同蜜蜂宿主的肠道微生物群在其抗生素耐药性结构方面存在差异，突出了蜜蜂微生物群作为抗生素耐药性潜在生物指示剂和传播者的作用。驯化历史的差异对蜜蜂肠道抗性基因组的结构有很大影响。值得注意的是，质粒介导的抗生素耐药性的演变可能会促进其持久性和传播的可能性。视频摘要在线版本包含补充材料，可通过10. 1186/s40168-022-01268-1获取。
The spread of antibiotic resistance genes (ARGs) has been of global concern as one of the greatest environmental threats. The gut microbiome of animals has been found to be a large reservoir of ARGs, which is also an indicator of the environmental antibiotic spectrum. The conserved microbiota makes the honeybee a tractable and confined ecosystem for studying the maintenance and transfer of ARGs across gut bacteria. Although it has been found that honeybee gut bacteria harbor diverse sets of ARGs, the influences of environmental variables and the mechanism driving their distribution remain unclear. We characterized the gut resistome of two closely related honeybee species, Apis cerana and Apis mellifera, domesticated in 14 geographic locations across China. The composition of the ARGs was more associated with host species rather than with geographical distribution, and A. mellifera had a higher content of ARGs in the gut. There was a moderate geographic pattern of resistome distribution, and several core ARG groups were found to be prevalent among A. cerana samples. These shared genes were mainly carried by the honeybee-specific gut members Gilliamella and Snodgrassella. Transferrable ARGs were frequently detected in honeybee guts, and the load was much higher in A. mellifera samples. Genomic loci of the bee gut symbionts containing a streptomycin resistance gene cluster were nearly identical to those of the broad-host-range IncQ plasmid, a proficient DNA delivery system in the environment. By in vitro conjugation experiments, we confirmed that the mobilizable plasmids could be transferred between honeybee gut symbionts by conjugation. Moreover, “satellite plasmids” with fragmented genes were identified in the integrated regions of different symbionts from multiple areas. Our study illustrates that the gut microbiota of different honeybee hosts varied in their antibiotic resistance structure, highlighting the role of the bee microbiome as a potential bioindicator and disseminator of antibiotic resistance. The difference in domestication history is highly influential in the structuring of the bee gut resistome. Notably, the evolution of plasmid-mediated antibiotic resistance is likely to promote the probability of its persistence and dissemination. Video Abstract The online version contains supplementary material available at 10.1186/s40168-022-01268-1.
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影响因子：
15.5
作者：
Cao, Jian;Hu, Yongfei;Gao, George F.
通讯作者：
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影响因子：
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