Development and regeneration of wheat-rice hybrid zygotes produced by in vitro fertilization system.
Development and regeneration of wheat-rice hybrid zygotes produced by in vitro fertilization system.
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DOI：
10.1111/nph.17747
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发表时间：
2021-12
期刊：
影响因子：
9.4
通讯作者：
Okamoto, Takashi
中科院分区：
文献类型：
--
作者：
Maryenti, Tety;Ishii, Takayoshi;Okamoto, Takashi
关键词：
杂交在被子植物的进化和多样化中起着决定性的作用。然而，广泛杂交的机制仍然是开放的，因为受精前和受精后的障碍分别限制了亚科间/属间合子的产生和发育。我们研究了小麦和水稻之间的杂交使用体外受精（IVF）系统，以绕过这些障碍。通过对异源多倍体麦稻杂种合子的研究，成功地获得了几个配子组合，并对杂种合子的发育特征进行了分析。来自一个水稻卵细胞和一个小麦精细胞的杂种合子在多细胞胚状结构阶段停止。这种发育障碍是通过在小麦-水稻杂交合子中加入一个小麦卵细胞而克服的。在一个小麦卵细胞和一个水稻精细胞的正反组合中，所产生的杂种合子不能分裂。然而，加倍水稻精细胞的剂量允许杂交合子发育成植株。水稻染色体在杂种胚的早期发育过程中逐渐消失，c。20%的再生植株出现形态异常。这些结果表明，杂交失败可以通过优化配子组合来克服，本杂交种将为亚家族间遗传资源的利用提供新的视野。
Hybridization plays a decisive role in the evolution and diversification of angiosperms. However, the mechanisms of wide hybridization remain open because pre‐ and post‐fertilization barriers limit the production and development of inter‐subfamily/intergeneric zygotes, respectively. We examined hybridization between wheat and rice using an in vitro fertilization (IVF) system to bypass these barriers. Several gamete combinations of allopolyploid wheat–rice hybrid zygotes were successfully produced, and the developmental profiles of hybrid zygotes were analyzed. Hybrid zygotes derived from one rice egg cell and one wheat sperm cell ceased at the multicellular embryo‐like structure stage. This developmental barrier was overcome by adding one wheat egg cell to the wheat–rice hybrid zygote. In the reciprocal combination, one wheat egg and one rice sperm cell, the resulting hybrid zygotes failed to divide. However, doubling the dosage of rice sperm cell allowed the hybrid zygotes to develop into plantlets. Rice chromosomes appeared to be progressively eliminated during the early developmental stage of these hybrid embryos, and c. 20% of regenerated plants showed abnormal morphology. These results suggest that hybrid breakdown can be overcome through optimization of gamete combinations, and the present hybrid will provide a new horizon for utilization of inter‐subfamily genetic resources.
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影响因子：
1.1
作者：
Komeda, Norio;Chaudhar, Harinder K.;Mukai, Yasuhiko
通讯作者：
Mukai, Yasuhiko
影响因子：
6.9
作者：
Abiko M;Maeda H;Tamura K;Hara-Nishimura I;Okamoto T
通讯作者：
Okamoto T
DOI：
10.1007/bf00391215
发表时间：
1926-01-01
期刊：
ZEITSCHR ZELLFORSCH U MIKROSK ANAT
影响因子：
--
作者：
KIHARA, H.;ONO, T.
通讯作者：
ONO, T.
影响因子：
64.8
作者：
Khanday, Imtiyaz;Skinner, Debra;Sundaresan, Venkatesan
通讯作者：
Sundaresan, Venkatesan
影响因子：
16.6
作者：
Denninger, Philipp;Bleckmann, Andrea;Lausser, Andreas;Vogler, Frank;Ott, Thomas;Ehrhardt, David W.;Frommer, Wolf B.;Sprunck, Stefanie;Dresselhaus, Thomas;Grossmann, Guido
通讯作者：
Grossmann, Guido