The pheromone-induced nuclear accumulation of the Fus3 MAPK in yeast depends on its phosphorylation state and on Dig1 and Dig2.
The pheromone-induced nuclear accumulation of the Fus3 MAPK in yeast depends on its phosphorylation state and on Dig1 and Dig2.
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DOI：
10.1186/1471-2121-8-44
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发表时间：
2007-10-26
期刊：
影响因子：
--
通讯作者：
Stone, David E.
中科院分区：
文献类型：
--
作者：
Blackwell, Ernest;Kim, Hye-Jin N.;Stone, David E.
与哺乳动物MAP激酶一样，酵母的交配特异性Fus 3 MAPK在受刺激细胞的细胞核中积累。由于Fus 3似乎不受积极的核质运输，目前还不清楚如何激活交配信息素的影响所观察到的变化，其本地化。一种可能性是Fus 3的激活改变了其对细胞核和细胞质系链的亲和力。Dig 1、Dig 2和Ste 12是与Fus 3相互作用的核蛋白。我们发现，在缺乏Dig 1或Dig 2的细胞中，信息素诱导的Fus 3-GFP报告基因的核积累减少，而Fus 3 T180 AY 182 A-GFP定位不受这些蛋白的缺乏的影响。这表明Dig 1和Dig 2有助于磷酸化的Fus 3在细胞核中的保留。此外，Ste 12的过表达导致Fus 3-GFP（但不是Fus 3 T180 AY 182 A-GFP）在信息素处理的细胞的细胞核中的过度积累，这表明Ste 12也在磷酸化Fus 3的核保留中起作用，或者通过与其直接相互作用，或者通过转录其蛋白质产物是Fus 3系链的基因。我们以前曾报道过，Msg 5磷酸酶的过表达抑制核定位的Fus 3。在这里，我们表明，这种效果取决于Msg 5的磷酸酶活性，并提供证据表明，细胞核和细胞质Msg 5可以影响Fus 3的本地化。我们的数据与信息素诱导的Fus 3磷酸化增加其对核系链的亲和力的模型一致，这有助于其核积累并被Msg 5拮抗。
Like mammalian MAP kinases, the mating-specific Fus3 MAPK of yeast accumulates in the nuclei of stimulated cells. Because Fus3 does not appear to be subjected to active nucleo-cytoplasmic transport, it is not clear how its activation by mating pheromone effects the observed change in its localization. One possibility is that the activation of Fus3 changes its affinity for nuclear and cytoplasmic tethers. Dig1, Dig2, and Ste12 are nuclear proteins that interact with Fus3. We found that the pheromone-induced nuclear accumulation of a Fus3-GFP reporter is reduced in cells lacking Dig1 or Dig2, whereas Fus3T180AY182A-GFP localization was unaffected by the absence of these proteins. This suggests that Dig1 and Dig2 contribute to the retention of phosphorylated Fus3 in the nucleus. Moreover, overexpression of Ste12 caused the hyper-accumulation of Fus3-GFP (but not Fus3T180AY182A-GFP) in the nuclei of pheromone-treated cells, suggesting that Ste12 also plays a role in the nuclear retention of phosphorylated Fus3, either by directly interacting with it or by transcribing genes whose protein products are Fus3 tethers. We have previously reported that overexpression of the Msg5 phosphatase inhibits the nuclear localization of Fus3. Here we show that this effect depends on the phosphatase activity of Msg5, and provide evidence that both nuclear and cytoplasmic Msg5 can affect the localization of Fus3. Our data are consistent with a model in which the pheromone-induced phosphorylation of Fus3 increases its affinity for nuclear tethers, which contributes to its nuclear accumulation and is antagonized by Msg5.
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影响因子：
16
作者：
Alepuz, PM;Jovanovic, A;Ammerer, G
通讯作者：
Ammerer, G
DOI：
10.1073/pnas.86.15.5703
发表时间：
1989-08-01
影响因子：
11.1
作者：
DOLAN, JW;KIRKMAN, C;FIELDS, S
通讯作者：
FIELDS, S
影响因子：
10.5
作者：
ERREDE, B;AMMERER, G
通讯作者：
AMMERER, G
DOI：
10.1073/pnas.95.26.15400
发表时间：
1998-12-22
影响因子：
11.1
作者：
Bardwell, L;Cook, JG;Thorner, J
通讯作者：
Thorner, J
影响因子：
10.5
作者：
Bardwell, L;Cook, JG;Thorner, J
通讯作者：
Thorner, J