Genome-wide mapping of Piwi association with specific loci in Drosophila ovaries.
Genome-wide mapping of Piwi association with specific loci in Drosophila ovaries.
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DOI：
10.1093/g3journal/jkaa059
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发表时间：
2021-02-09
期刊：
影响因子：
--
通讯作者：
Lin H
中科院分区：
文献类型：
--
作者：
Liu N;Neuenkirchen N;Zhong M;Lin H
小的非编码RNA通路涉及多种基因调控机制。在果蝇卵巢中，Piwi与Piwi相互作用的rna （piRNAs）结合，主要是24-28个核苷酸（nt），在种系干细胞维持、转座子抑制和表观遗传调控中发挥重要作用。为了了解这些功能背后的机制，我们报道了DamID-seq方法在果蝇卵巢中鉴定Piwi全基因组结合位点的应用。Piwi定位于至少4535个富含piRNA靶位点的共染色质区域。令人惊讶的是，Piwi与常染色质结合的密度比异染色质高得多。破坏Piwi的piRNA结合会导致基因组结合谱的整体变化，这表明piRNA在引导Piwi到达特定基因组位点方面的作用。大多数Piwi结合位点位于蛋白质编码基因内部或附近，特别是在转录起始和终止位点附近富集。当Piwi突变成为piRNA结合缺陷时，转录终止位点附近的甲基化信号显著减少。这些观察结果表明，Piwi可能直接调节许多蛋白质编码基因的表达，特别是通过调节目标转录物的3'端。
Small noncoding RNA pathways have been implicated in diverse mechanisms of gene regulation. In Drosophila ovaries, Piwi binds to Piwi-interacting RNAs (piRNAs) of mostly 24–28 nucleotides (nt) and plays an important role in germline stem cell maintenance, transposon repression, and epigenetic regulation. To understand the mechanism underlying these functions, we report the application of the DamID-seq method to identify genome-wide binding sites of Piwi in Drosophila ovaries. Piwi localizes to at least 4535 euchromatic regions that are enriched with piRNA target sites. Surprisingly, the density of Piwi binding to euchromatin is much higher than in heterochromatin. Disrupting the piRNA binding of Piwi results in an overall change of the genomic binding profile, which indicates the role of piRNAs in directing Piwi to specific genomic sites. Most Piwi binding sites were either within or in the vicinity of protein-coding genes, particularly enriched near the transcriptional start and termination sites. The methylation signal near the transcriptional termination sites is significantly reduced when Piwi was mutated to become defective in piRNA binding. These observations indicate that Piwi might directly regulate the expression of many protein-coding genes, especially through regulating the 3' ends of targeted transcripts.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
11.8
作者：
Marinov, Georgi K.;Wang, Jie;Handler, Dominik;Wold, Barbara J.;Weng, Zhiping;Hannon, Gregory J.;Aravin, Alexei A.;Zamore, Phillip D.;Brennecke, Julius;Toth, Katalin Fejes
通讯作者：
Toth, Katalin Fejes
影响因子：
30.8
作者：
Peng JC;Valouev A;Liu N;Lin H
通讯作者：
Lin H
影响因子：
30.8
作者：
Gangaraju, Vamsi K.;Yin, Hang;Weiner, Molly M.;Wang, Jianquan;Huang, Xiao A.;Lin, Haifan
通讯作者：
Lin, Haifan
Piwi与核孔处的染色质相互作用，并混合结合果蝇卵巢体细胞中的核转录本。
影响因子：
14.9
作者：
Ilyin AA;Ryazansky SS;Doronin SA;Olenkina OM;Mikhaleva EA;Yakushev EY;Abramov YA;Belyakin SN;Ivankin AV;Pindyurin AV;Gvozdev VA;Klenov MS;Shevelyov YY
通讯作者：
Shevelyov YY