An S-adenosyl Methionine Synthetase (SAMS) Gene from Andropogon virginicus L. Confers Aluminum Stress Tolerance and Facilitates Epigenetic Gene Regulation in Arabidopsis thaliana.
An S-adenosyl Methionine Synthetase (SAMS) Gene from Andropogon virginicus L. Confers Aluminum Stress Tolerance and Facilitates Epigenetic Gene Regulation in Arabidopsis thaliana.
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DOI：
10.3389/fpls.2016.01627
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发表时间：
2016
影响因子：
5.6
通讯作者：
Nishiuchi T
中科院分区：
文献类型：
--
作者：
Ezaki B;Higashi A;Nanba N;Nishiuchi T
从高铝耐受性的禾科植物Andropogon virginicus L.的cDNA文库中获得了对酵母转化子（TFs）具有铝耐受性的候选克隆，其中AL3A-4克隆编码一个s -腺苷型蛋氨酸合成酶（SAMS）基因。利用5′-RACE技术获得全长cDNA，命名为AvSAMS1，并将其导入拟南芥，研究其在铝胁迫下的生物学功能。2个TF株系均表现出比Col-0生态型（非TF）更高的耐Al、Cu、Pb、Zn和二胺等逆境胁迫的能力，表明AvSAMS1是一个多重耐受性基因。从芯片结果中选择了40多个拟沙蚕Al反应基因（Al诱导基因和Al抑制基因），用于研究col0和AvSAMS1 TF系在Al胁迫下的DNA或组蛋白甲基化状态。结果表明，铝胁迫引起DNA和组蛋白H3 （H3K4me3和H3K9me3）甲基化状态的改变，并且这些改变在AvSAMS1 TF和Col-0之间是不同的，表明这些差异是AvSAMS1基因依赖性的。这些结果提示在铝胁迫下存在与avsams1相关的表观遗传基因调控。
Candidate clones which conferred Al tolerance to yeast transformants (TFs) were obtained from a cDNA library derived from a highly Al-tolerant poaceae, Andropogon virginicus L. One such clone, AL3A-4, encoded an S-adenosyl methionine synthetase (SAMS) gene. A full-length cDNA was obtained by 5′-RACE, designated AvSAMS1, and introduced into Arabidopsis thaliana to investigate its biological functions under Al stress. Two TF plant lines both showed higher tolerance than the Col-0 ecotype (non-TF) not only for Al stress, but also for Cu, Pb, Zn and diamide stresses, suggesting the AvSAMS1 was a multiple tolerance gene. More than 40 of A. thaliana Al response-genes (Al induced genes and Al repressed genes) were selected from microarray results and then used for investigations of DNA or histone methylation status under Al stress in Col-0 and the AvSAMS1 TF line. The results indicated that Al stress caused alterations of methylation status in both DNA and histone H3 (H3K4me3 and H3K9me3) and that these alterations were different between the AvSAMS1 TF and Col-0, suggesting the differences were AvSAMS1-gene dependent. These results suggested the existence of AvSAMS1-related epigenetic gene-regulation under Al stress.
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与干旱对比和盐度应激反应的水稻品种中与基因表达相关的不同DNA甲基化模式。
影响因子：
4.6
作者：
Garg R;Narayana Chevala V;Shankar R;Jain M
通讯作者：
Jain M
DOI：
10.1016/j.bbagrm.2011.03.007
发表时间：
2011-08
影响因子：
4.7
作者：
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通讯作者：
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影响因子：
6.9
作者：
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影响因子：
7.4
作者：
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通讯作者：
RANDALL, PJ
影响因子：
6.9
作者：
Ellis, RP;Forster, BP;Powell, W
通讯作者：
Powell, W