Highly sensitive AlGaN/GaN HEMT biosensors using an ethanolamine modification strategy for bioassay applications.
Highly sensitive AlGaN/GaN HEMT biosensors using an ethanolamine modification strategy for bioassay applications.
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采用乙醇胺修饰策略的高灵敏度 AlGaN/GaN HEMT 生物传感器，用于生物测定应用
作者：
在本文中，我们提出了一种高效的AlGaN/GaN高电子迁移率晶体管（HEMT）的表面改性策略，其中乙醇胺（EA）被用来功能化的GaN表面，并提供两性胺基团的探针分子固定的生物测定应用。利用分子门控AlGaN/GaN HEMT进行pH和前列腺特异性抗原（PSA）检测，以验证其作为生物传感器的性能。受益于GaN表面的高涂层质量，我们的生物传感器的性能相比之前报道的其他AlGaN/GaN HEMT基pH和PSA生物传感器有了大幅提高。我们研制的分子门控AlGaN/GaN HEMT生物传感器具有灵敏度高、线性度好、化学稳定性好等优良的静态电学性能。此外，在将PSA抗体进一步固定到EA胺化的GaN表面上之后，PSA检测的检测限（LOD）在PBS缓冲液中低至1fg mL-1，这与之前报道的任何其他基于AlGaN/GaN HEMT的PSA生物传感器相比已经达到至少两个数量级的降低。我们的PSA生物传感器的灵敏度大幅提高，达到2.04%（100 ng mL−1）。利用EA修饰的AlGaN/GaNHEMT生物传感器进行pH和PSA的测量，结果表明本文提出的GaN表面修饰策略可以有效地提高AlGaN/GaNHEMT生物传感器的性能，在AlGaN/GaNHEMT生物检测领域具有良好的应用前景。
In this paper, we propose a highly efficient surface modification strategy on an AlGaN/GaN high electron mobility transistor (HEMT), where ethanolamine (EA) was utilized to functionalize the surface of GaN and provided amphoteric amine groups for probe molecular immobilization for bioassay application. The molecular gated-AlGaN/GaN HEMT was utilized for pH and prostate-specific antigen (PSA) detection to verify its performance as a biosensor. Benefitting from the high coating quality on the GaN surface, the performance of our biosensor is drastically improved compared to other AlGaN/GaN HEMT based pH and PSA biosensors reported before. Our molecular gated-AlGaN/GaN HEMT biosensor has achieved good static electrical performance for pH sensing, such as high sensitivity, good linearity and chemical stability. Moreover, after further immobilization of PSA antibody onto the EA aminated GaN surface, the limit of detection (LOD) for PSA detection is as low as 1 fg mL−1 in PBS buffer, which has reached an at least two orders of magnitude decrease compared to any other AlGaN/GaN HEMT based PSA biosensor reported before. And the sensitivity of our PSA biosensor has achieved a substantial increase, reaching up to 2.04% for 100 ng mL−1. The measurements of pH and PSA utilizing the EA modified AlGaN/GaN HEMT biosensor indicate that the surface modification strategy on the GaN proposed in this paper can effectively improve the performance of the AlGaN/GaN HEMT based biosensor, which demonstrates a promising application prospect in the AlGaN/GaN HEMT based biological detection field.
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