Lactobacillus casei SYF-08 Protects Against Pb-Induced Injury in Young Mice by Regulating Bile Acid Metabolism and Increasing Pb Excretion.
Lactobacillus casei SYF-08 Protects Against Pb-Induced Injury in Young Mice by Regulating Bile Acid Metabolism and Increasing Pb Excretion.
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干酪乳杆菌 SYF-08 通过调节胆汁酸代谢和增加铅排泄来保护幼鼠免受铅引起的损伤
DOI：
10.3389/fnut.2022.914323
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发表时间：
2022
影响因子：
5
通讯作者：
Fan, Hongying
中科院分区：
文献类型：
--
作者：
Chen, Zhenhui;Tang, Ziyu;Kong, Jingjing;Chen, Lixuan;Liu, Jiaxin;Li, Yunting;Huang, Wanwen;Li, Wendan;Wu, Junlin;Zhao, Wei;Meng, Xiaojing;Fan, Hongying
关键词：
铅中毒影响婴儿生长发育。然而，目前治疗铅中毒的二巯基丁二酸（DMSA）对婴儿的毒副作用较大。因此，寻找一种无毒的治疗方法就显得尤为重要。在本研究中，我们旨在研究婴儿粪便益生菌菌株干酪乳杆菌 SYF-08 (SYF-08) 对幼鼠铅中毒的治疗作用。通过电感耦合等离子体质谱法检测生物体中的Pb水平，并通过Morris水迷宫试验、苏木精-伊红染色和免疫组织化学探讨SYF-08对Pb诱导的神经系统损伤的治疗作用。此外，还通过组织学染色、16S rRNA测序、非靶向代谢组学、qRT-PCR和蛋白质印迹法探索了SYF-08对Pb诱导的肠道损伤的保护作用的分子机制。体内实验表明，SYF-08 降低了血液和骨骼中的 Pb 水平，并增加了尿液中 Pb 的排泄。此外，SYF-08减轻了铅引起的大脑病理损伤，最终提高了幼鼠的学习和认知能力。这种治疗还恢复了肠道菌群失调，调节胆汁酸代谢，并抑制 FXR-NLRP3 信号通路。与 DMSA 治疗相比，它还导致更少的不良事件。总之，我们的结果为 SYF-08 在铅中毒中的治疗作用提供了有价值的见解，并表明其给药可以显着减轻铅引起的损伤。
Pb poisoning affects infant growth and development. However, dimercaptosuccinic acid (DMSA) as the current therapy for Pb poisoning exerts relatively significant toxic side effects in infants. Therefore, identifying a non-toxic treatment in this regard is particularly important. In this study, we aimed to investigate the therapeutic effect of an infant feces-derived probiotic strain, Lactobacillus casei SYF-08 (SYF-08), on Pb poisoning in young mice. The Pb levels in the organisms were detected via inductively coupled plasma mass spectrometry, while the therapeutic effect of SYF-08 on Pb-induced neural system damage was explored via the Morris water maze test, hematoxylin-eosin staining, and immunohistochemistry. Additionally, the molecular mechanisms underlying the protective effects of SYF-08 against Pb-induced intestinal damage were also explored via histological staining, 16S rRNA sequencing, untargeted metabolomics, qRT-PCR, and western blotting. In vivo experiments revealed that SYF-08 reduced blood and bone Pb levels and increased urinary Pb excretion. Additionally, SYF-08 alleviated Pb-induced pathological damage to the brain and ultimately improved the learning and cognitive abilities of the young mice. This treatment also restored intestinal microflora dysbiosis, regulated bile acid metabolism, and inhibited the FXR-NLRP3 signaling pathway. It also resulted in fewer adverse events than the DMSA treatment. In conclusion, our results provided valuable insights into the therapeutic role of SYF-08 in Pb poisoning and also suggested that its administration can significantly alleviate the Pb-induced damage.
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DOI：
10.3390/ijerph18073551
发表时间：
2021-03-29
影响因子：
--
作者：
Bud ES;Bica CI;Stoica OE;Vlasa A;Eșian D;Bucur SM;Bud A;Chibelean M;Păcurar M
通讯作者：
Păcurar M
影响因子：
6.4
作者：
Garneau JE;Moineau S
通讯作者：
Moineau S
影响因子：
29
作者：
Clifford BL;Sedgeman LR;Williams KJ;Morand P;Cheng A;Jarrett KE;Chan AP;Brearley-Sholto MC;Wahlström A;Ashby JW;Barshop W;Wohlschlegel J;Calkin AC;Liu Y;Thorell A;Meikle PJ;Drew BG;Mack JJ;Marschall HU;Tarling EJ;Edwards PA;de Aguiar Vallim TQ
通讯作者：
de Aguiar Vallim TQ
德氏乳杆菌保加利亚亚种KLDS1.0207缓解铅毒性作用的体外和体内评价
影响因子：
5.9
作者：
Li B;Jin D;Yu S;Etareri Evivie S;Muhammad Z;Huo G;Liu F
通讯作者：
Liu F
影响因子：
5.8
作者：
Edgar, Robert C.;Flyvbjerg, Henrik
通讯作者：
Flyvbjerg, Henrik