Human adipose tissue macrophages are enhanced but changed to an anti-inflammatory profile in obesity.
Human adipose tissue macrophages are enhanced but changed to an anti-inflammatory profile in obesity.
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DOI：
10.1155/2014/309548
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发表时间：
2014
影响因子：
4.1
通讯作者：
Richelsen B
中科院分区：
文献类型：
--
作者：
Fjeldborg K;Pedersen SB;Møller HJ;Christiansen T;Bennetzen M;Richelsen B
客观的。肥胖时脂肪组织 (AT) 巨噬细胞增多，并与轻度炎症相关。我们的目的是表征人类 AT 巨噬细胞的表型与肥胖和胰岛素抵抗的关系。设计。通过RT-PCR测定瘦和肥胖受试者皮下AT样本中一般巨噬细胞标记物（CD68和CD14）、促炎标记物/M1（TNF-α、MCP-1和IL-6）和抗炎标记物/M2（CD163、CD206和IL-10）的基因表达水平。通过 HOMA-IR 测定胰岛素抵抗。结果。与瘦受试者相比，肥胖受试者的 AT 中所有巨噬细胞标记物均升高（P < 0.001）。为了确定巨噬细胞的表型，使用 CD14 的水平来调整巨噬细胞的总数。肥胖受试者AT中CD163和IL-10的相对表达升高，TNF-α和IL-6的相对表达降低（均P < 0.05）。在多变量回归分析中，CD163 是唯一与 HOMA-IR 显着相关的巨噬细胞标记物（β：0.57；P < 0.05）。结论。肥胖与 AT 中巨噬细胞数量增加有关。出乎意料的是，巨噬细胞因肥胖而改变表型，肥胖受试者的 AT 中 M2 标记物占优势，M1 标记物减少。而且，经过多次调整，CD163是唯一与HOMA-IR相关的巨噬细胞标志物。
Objective. Adipose tissue (AT) macrophages are increased in obesity and associated with low grade inflammation. We aimed to characterize the phenotype of AT macrophages in humans in relation to obesity and insulin resistance. Design. Gene-expression levels of general macrophage markers (CD68 and CD14), proinflammatory markers/M1 (TNF-α, MCP-1, and IL-6), and anti-inflammatory markers/M2 (CD163, CD206, and IL-10) were determined by RT-PCR in subcutaneous AT samples from lean and obese subjects. Insulin resistance was determined by HOMA-IR. Results. All the macrophage markers were elevated in the AT from obese compared to lean subjects (P < 0.001). To determine the phenotype of the macrophages the level of CD14 was used to adjust the total number of macrophages. The relative expression of CD163 and IL-10 was elevated, and TNF-α and IL-6 were reduced in AT from obese subjects (all P < 0.05). In a multivariate regression analysis CD163 was the only macrophage marker significantly associated with HOMA-IR (β: 0.57; P < 0.05). Conclusion. Obesity is associated with elevated numbers of macrophages in the AT. Unexpectedly, the macrophages change phenotype by obesity, with a preponderance of M2 and a decrement of M1 markers in AT from obese subjects. Moreover, CD163 was the only macrophage marker associated with HOMA-IR after multiple adjustments.
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影响因子：
20.3
作者：
Moller, HJ;Peterslund, NA;Moestrup, SK
通讯作者：
Moestrup, SK
影响因子：
4.8
作者：
Clément, K;Viguerie, N;Langin, D
通讯作者：
Langin, D
影响因子：
5.8
作者：
Aron-Wisnewsky, Judith;Tordjman, Joan;Clement, Karine
通讯作者：
Clement, Karine
影响因子：
29
作者：
Bouhlel, M. Amine;Derudas, Bruno;Chinetti-Gbaguidi, Giulia
通讯作者：
Chinetti-Gbaguidi, Giulia
影响因子：
3
作者：
Arvidsson S;Kwasniewski M;Riaño-Pachón DM;Mueller-Roeber B
通讯作者：
Mueller-Roeber B