Small Noncoding RNAs Contribute to Sperm Oxidative Stress-Induced Programming of Behavioral and Metabolic Phenotypes in Offspring.
Small Noncoding RNAs Contribute to Sperm Oxidative Stress-Induced Programming of Behavioral and Metabolic Phenotypes in Offspring.
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小非编码 RNA 有助于精子氧化应激诱导的后代行为和代谢表型编程
DOI：
10.1155/2022/6877283
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发表时间：
2022
影响因子：
--
通讯作者：
He, Bin
中科院分区：
文献类型：
--
作者：
Ren, Li;Xin, Yining;Sun, Xiaoxiao;Zhang, Yanwen;Chen, Yingqi;Liu, Suyuan;He, Bin
越来越多的证据表明，父亲的环境信息会改变精子中的小非编码rna (sncRNAs)，进而诱导下一代代谢和行为表型的改变。然而，潜在的介质的影响仍有待阐明。多种多样的环境损害和胁迫可以共同诱导精子中活性氧（ROS）的升高；然而，目前尚不清楚ROS是否介导精子中sncrna的生物发生并参与后代表型的重编程。在这里，我们发现ROS可以诱导精子中sncRNA谱的改变，特别是对于转移rna衍生的小rna （tsRNAs）和核糖体rna衍生的小rna （rsRNAs）。从氧化应激（OS）精子中注射29-34 nt RNA片段（主要为tsrna和rsRNAs）可诱导雄性后代出现抑郁样和焦虑样行为。此外，合成rna的受精卵注射部分类似于OS精子诱导的后代抑郁样和焦虑样行为。研究发现，以鼠粮喂养的雄性后代会出现糖耐量受损和肝糖异生过度活跃，并伴有肝糖异生和脂溶基因的上调。总之，我们的研究结果表明，ros诱导的精子中sncRNA谱的改变有助于后代行为和代谢表型的改变。
There is growing evidence that paternal environmental information alters small noncoding RNAs (sncRNAs) in sperm and in turn can induce alterations of metabolic and behavioral phenotypes of the next generation. However, the potential mediators of the effects remain to be elucidated. A great diversity of environmental insults and stresses can convergently induce the elevation of reactive oxygen species (ROS) in sperm; nonetheless, it remains unclear whether ROS mediates the biogenesis of sncRNAs in sperm and participates in the reprogramming of offspring phenotypes. Here, we show that ROS could induce the alteration of sncRNA profiles in sperm, especially for transfer RNA-derived small RNAs (tsRNAs) and ribosomal RNA-derived small RNAs (rsRNAs). Zygotic injection of 29-34 nt RNA fractions (predominantly tsRNAs and rsRNAs) from oxidative stress (OS) sperm could induce depressive-like and anxiety-like behaviors in male offspring. Moreover, zygotic injection with synthetic RNAs partially resembled OS sperm-induced depressive-like and anxiety-like behaviors in offspring. Male offspring maintained on a chow diet was found to develop impaired glucose tolerance and hyperactive hepatic gluconeogenesis, accompanied by the upregulation of hepatic gluconeogenic and lipolytic genes. Together, our results have shown that ROS-induced alteration of sncRNA profiles in sperm contributes to the alterations of behavioral and metabolic phenotypes of the offspring.
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影响因子：
64.5
作者：
Carone BR;Fauquier L;Habib N;Shea JM;Hart CE;Li R;Bock C;Li C;Gu H;Zamore PD;Meissner A;Weng Z;Hofmann HA;Friedman N;Rando OJ
通讯作者：
Rando OJ
影响因子：
25
作者：
Gapp, Katharina;Jawaid, Ali;Sarkies, Peter;Bohacek, Johannes;Pelczar, Pawel;Prados, Julien;Farinelli, Laurent;Miska, Eric;Mansuy, Isabelle M.
通讯作者：
Mansuy, Isabelle M.
DOI：
10.1073/pnas.1803630115
发表时间：
2018-10-30
影响因子：
11.1
作者：
Costa DL;Yetter N;DeSomer H
通讯作者：
DeSomer H
通过转录组和小 RNA 测序探索新鲜和冻融公猪精子中的 miRNA 和 mRNA 谱。
影响因子：
5.6
作者：
Dai, Ding-Hui;Qazi, Izhar Hyder;Zeng, Chang-Jun
通讯作者：
Zeng, Chang-Jun
影响因子：
2.4
作者：
Cicare, J.;Caille, A.;Munuce, M. J.
通讯作者：
Munuce, M. J.