Direct Speciation Analysis of Arsenic in Whole Blood and Blood Plasma at Low Exposure Levels by Hydride Generation-Cryotrapping-Inductively Coupled Plasma Mass Spectrometry.
Direct Speciation Analysis of Arsenic in Whole Blood and Blood Plasma at Low Exposure Levels by Hydride Generation-Cryotrapping-Inductively Coupled Plasma Mass Spectrometry.
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DOI：
10.1021/acs.analchem.7b01868
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发表时间：
2017-09-19
影响因子：
7.4
通讯作者：
Stýblo M
中科院分区：
文献类型：
--
作者：
Matoušek T;Wang Z;Douillet C;Musil S;Stýblo M
采用低温诱捕选择性氢化物生成（HG-CT）与石英多雾化器原子吸收光谱法（AAS）或电感耦合等离子体质谱法（ICPMS）对血浆和全血中重要的毒理学砷进行了分析。样品制备只需要5倍稀释，加入Triton X- 100， Antifoam B和l -半胱氨酸，就可以抑制氢化物发生器中的过度起泡。AAS和ICPMS检测中，在0.25 ~ 1 μg L−1砷酸盐和0.025 ~ 0.1 μg L−1砷酸盐浓度下的全血和血浆的校准斜率与水溶液的校准斜率没有差异。HG-CT-AAS用于分析砷含量升高的样品——急性早幼粒细胞白血病患者接受三氧化二砷治疗的血浆和喂食含砷饮食的小鼠的全血。直接分析结果与在磷酸中温和消化后的分析结果吻合良好，证明了直接HG-CT方法对这类生物样品中的砷种类具有良好的效率。下一步，用直接HG-CT-ICPMS分析健康献血者的血浆和全血，白血病患者的血浆浓度为0.15 - 0.4 μg L−1 As。即使在这种低水平下，所有物种的回收率也很好（88 ~ 104%）。血液和血浆中无机砷的检出限为0.014 μg L−1，甲基化砷的检出限低于0.002 μg L−1。因此，超灵敏的直接HG-CT-ICPMS方法特别适用于低暴露水平个体的血浆和全血分析。
A method for analysis of toxicologically important arsenic species in blood plasma and whole blood by selective hydride generation with cryotrapping (HG-CT) coupled either to atomic absorption spectrometry (AAS) with a quartz multiatomizer or to inductively coupled plasma mass spectrometry (ICPMS) has been validated. Sample preparation, which involved only 5 times dilution with addition of Triton X- 100, Antifoam B, and L-cysteine, suppressed excessive foaming in a hydride generator. Calibration slopes for whole blood and blood plasma spiked with arsenate, monomethylarsonate, and dimethylarsinate at 0.25−1 μg L−1 As and 0.025−0.1 μg L−1 As for AAS and ICPMS detection, respectively, did not differ from slopes in aqueous solutions. HG-CT-AAS was used to analyze samples with elevated levels of arsenic species-blood plasma from patients treated with arsenic trioxide for acute promyelocytic leukemia and whole blood from mice fed an arsenic-containing diet. A good agreement between results of the direct analysis and analysis after mild digestion in phosphoric acid proved the good efficiency of the direct HG-CT procedure for the arsenic species in these types of biological samples. In the next step, plasma and whole blood from healthy donors that were spiked with the plasma from leukemia patients at levels of 0.15−0.4 μg L−1 As were analyzed by direct HG-CT-ICPMS. Good recoveries for all species even at these low levels (88−104%) were obtained. Limits of detection in blood and plasma were 0.014 μg L−1 for inorganic arsenic and below 0.002 μg L−1 As for methylated arsenic species. Thus, the ultrasensitive direct HG-CT-ICPMS method is uniquely suited for analyses of blood plasma and whole blood from individuals at low exposure levels.
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影响因子：
3.4
作者：
Ito, Kanna;Palmer, Christopher D.;Parsons, Patrick J.
通讯作者：
Parsons, Patrick J.
影响因子：
10.4
作者：
Hall M;Gamble M;Slavkovich V;Liu X;Levy D;Cheng Z;van Geen A;Yunus M;Rahman M;Pilsner JR;Graziano J
通讯作者：
Graziano J
影响因子：
4.1
作者：
Currier, Jenna M.;Svoboda, Milan;Styblo, Miroslav
通讯作者：
Styblo, Miroslav
影响因子：
3.4
作者：
Hernandez-Zavala, Araceli;Matousek, Tomas;Styblo, Miroslav
通讯作者：
Styblo, Miroslav
影响因子：
3.3
作者：
Matousek, Tomas;Hernandez-Zavala, Araceli;Dedina, Jiri
通讯作者：
Dedina, Jiri