Novel xylanases from Simplicillium obclavatum MTCC 9604: comparative analysis of production, purification and characterization of enzyme from submerged and solid state fermentation.
Novel xylanases from Simplicillium obclavatum MTCC 9604: comparative analysis of production, purification and characterization of enzyme from submerged and solid state fermentation.
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DOI：
10.1186/2193-1801-2-382
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Ghosh S
中科院分区：
文献类型：
--
作者：
Roy S;Dutta T;Sarkar TS;Ghosh S
研究了一株新分离的真菌Simplicillium obclavatum MTCC 9604的固态发酵和液体发酵产胞外木聚糖酶的情况。该菌株在麸皮上生长时，固态发酵可产生多种木聚糖酶和内切葡聚糖酶。以麦麸为唯一碳源，采用液体深层发酵的方法，发现同一种真菌可产生单一的木聚糖酶异构体。SSF的酶活、稳定性和蛋白质得率均高于SF。该粗酶的二维酶谱表明存在6种同工型，从pH 3-10开始具有不同的pI值。部分纯化的木聚糖酶活性的最适温度和pH分别为50°C和pH5.0;木聚糖酶在较宽的pH范围和30-60°C的温度范围内表现出显著的稳定性，这在生物燃料、动物饲料和食品工业应用中具有巨大的潜力。
The production of extracellular xylanase by a newly isolated fungus Simplicillium obclavatum MTCC 9604 was studied in solid-state and submerged fermentation. Multiple xylanases and endoglucanases were produced by the strain during growth on wheat bran in solid state fermentation (SSF). A single xylanase isoform was found to be produced by the same fungus under submerged fermentation (SF) using wheat bran as sole carbon source. Enzyme activity, stability and the protein yield were much higher in SSF than SF. The two dimensional zymogram of the crude enzyme indicated the presence of six isoforms with different pI values starting from pH 3–10. The optimum temperature and pH for the partially purified xylanase activity were 50°C and pH 5.0 respectively; xylanase enzymes exhibited remarkable stability over a broad pH range and the temperature range of 30-60°C which has great potential to be used in biofuels, animal feed and food industry applications.
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