Two Potential Syphilis Vaccine Candidates Inhibit Dissemination of Treponema pallidum.
Two Potential Syphilis Vaccine Candidates Inhibit Dissemination of Treponema pallidum.
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DOI：
10.3389/fimmu.2021.759474
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发表时间：
2021
影响因子：
7.3
通讯作者：
Wu Y
中科院分区：
文献类型：
--
作者：
Xu M;Xie Y;Zheng K;Luo H;Tan M;Zhao F;Zeng T;Wu Y
梅毒螺旋体引起的梅毒螺旋体，梅毒亚种，仍然是一个重大的公共卫生问题，世界各地。最近梅毒病例的增加，加上缺乏有效的梅毒疫苗。人类梅毒，突出了一个有效的梅毒疫苗的设计和开发的迫切需要。在这里，我们评估了粘附蛋白TP0136和外膜蛋白TP0663的疫苗潜力。用重组蛋白Tp0136、Tp0663或对照PBS皮下免疫家兔。用Tp0136或Tp0663免疫产生强的体液免疫应答，具有高滴度的IgG，如通过ELISA评估的。此外，与未免疫的动物相比，用Tp 0136或Tp 0663免疫的动物表现出减弱的病变发展、在病变部位增加的细胞浸润和对密螺旋体向远端器官传播的抑制。这些发现表明，Tp 0136和Tp 0663是有希望的梅毒疫苗候选者。这些结果不仅为了解螺旋体的致病机制，而且为螺旋体特异性亚单位疫苗的开发提供了新的重要信息。
Syphilis, caused by the spirochete Treponema pallidum subspecies pallidum, continues to be a major public health problem worldwide. Recent increases in the number of syphilis cases, in addition to the lack of an efficient vaccine against T. pallidum for humans, highlights an urgent need for the design and development of an efficacious syphilis vaccine. Here, we assess the vaccine potential of the adhesion protein Tp0136 and the outer membrane protein Tp0663. Rabbits were subcutaneously immunized with recombinant proteins Tp0136, Tp0663, or control PBS. Immunization with Tp0136 or Tp0663 generated a strong humoral immune response with high titers of IgG, as assessed by ELISA. Moreover, animals immunized with Tp0136 or Tp0663 exhibited attenuated lesion development, increased cellular infiltration at the lesion sites, and inhibition of treponemal dissemination to distant organs compared to the unimmunized animals. These findings indicate that Tp0136 and Tp0663 are promising syphilis vaccine candidates. Furthermore, these results provide novel and important information for not only understanding the pathogenic mechanisms of spirochetes, but also the development of spirochete-specific subunit vaccines.
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影响因子：
3.8
作者：
Ke W;Molini BJ;Lukehart SA;Giacani L
通讯作者：
Giacani L
影响因子：
9.4
作者：
Brinkman, MB;McKevitt, M;Palzkill, T
通讯作者：
Palzkill, T
影响因子：
6.4
作者：
Cameron, CE;Lukehart, SA;Van Voorhis, WC
通讯作者：
Van Voorhis, WC
影响因子：
3.2
作者：
Champion, CI;Blanco, DR;Lovett, MA
通讯作者：
Lovett, MA
影响因子：
--
作者：
Lukehart, Sheila A;Marra, Christina M
通讯作者：
Marra, Christina M