The ins and outs of membrane bending by intrinsically disordered proteins.
The ins and outs of membrane bending by intrinsically disordered proteins.
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DOI：
10.1126/sciadv.adg3485
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发表时间：
2023-07-07
期刊：
影响因子：
13.6
通讯作者：
Stachowiak, Jeanne C.
中科院分区：
文献类型：
--
作者：
Yuan, Feng;Lee, Christopher T.;Sangani, Arjun;Houser, Justin R.;Wang, Liping;Lafer, Eileen M.;Rangamani, Padmini;Stachowiak, Jeanne C.
膜曲率对于多种细胞功能至关重要。虽然传统上归因于结构域，但最近的研究表明，本质上无序的蛋白质也是膜弯曲的有效驱动因素。具体来说，无序域之间的排斥相互作用驱动凸弯曲，而吸引相互作用驱动凹弯曲，产生膜结合的液体状冷凝物。同时包含排斥域和吸引域的无序域如何影响曲率？在这里，我们研究了结合了吸引和排斥相互作用的嵌合体。当吸引域更靠近膜时，其凝结会放大排斥域之间的空间压力，导致凸曲率。相反，当排斥域更接近膜时，吸引相互作用占主导地位，导致凹曲率。此外，随着离子强度的增加，发生从凸曲率到凹曲率的转变，这减少了排斥，同时增强了凝聚。与简单的机械模型一致，这些结果说明了无序蛋白质引起的膜弯曲的一组设计规则。一组设计规则揭示了无序蛋白质如何影响膜曲率。
Membrane curvature is essential to diverse cellular functions. While classically attributed to structured domains, recent work illustrates that intrinsically disordered proteins are also potent drivers of membrane bending. Specifically, repulsive interactions among disordered domains drive convex bending, while attractive interactions drive concave bending, creating membrane-bound, liquid-like condensates. How might disordered domains that contain both repulsive and attractive domains affect curvature? Here, we examined chimeras that combined attractive and repulsive interactions. When the attractive domain was closer to the membrane, its condensation amplified steric pressure among repulsive domains, leading to convex curvature. In contrast, when the repulsive domain was closer to the membrane, attractive interactions dominated, resulting in concave curvature. Further, a transition from convex to concave curvature occurred with increasing ionic strength, which reduced repulsion while enhancing condensation. In agreement with a simple mechanical model, these results illustrate a set of design rules for membrane bending by disordered proteins. A set of design rules reveals how disordered proteins can impact membrane curvature.
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DOI：
10.1016/j.bbamcr.2021.119205
发表时间：
2022-01-06
影响因子：
5.1
作者：
Nag, Niharika;Sasidharan, Santanu;Tripathi, Timir
通讯作者：
Tripathi, Timir
影响因子：
64.5
作者：
Hurley JH;Boura E;Carlson LA;Różycki B
通讯作者：
Różycki B
影响因子：
3.4
作者：
EVANS, EA
通讯作者：
EVANS, EA
影响因子：
19
作者：
Boeynaems S;Alberti S;Fawzi NL;Mittag T;Polymenidou M;Rousseau F;Schymkowitz J;Shorter J;Wolozin B;Van Den Bosch L;Tompa P;Fuxreiter M
通讯作者：
Fuxreiter M
影响因子：
3.4
作者：
Neal, BL;Asthagiri, D;Lenhoff, AM
通讯作者：
Lenhoff, AM