Factors Influencing Marker Expressions of Cultured Human Cord Blood-Derived Mast Cells.
Factors Influencing Marker Expressions of Cultured Human Cord Blood-Derived Mast Cells.
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DOI：
10.3390/ijms241914891
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发表时间：
2023-10-04
影响因子：
5.6
通讯作者：
Di Nardo A
中科院分区：
文献类型：
--
作者：
Alimohammadi S;Masuda-Kuroki K;Szöllősi AG;Di Nardo A
肥大细胞（MC）是造血来源的组织驻留免疫细胞，在先天性和适应性免疫中发挥重要作用。当补充有细胞因子如干细胞因子、白细胞介素3和白细胞介素6时，可以从各种组织来源（包括脐带血）分离和分化人MC。我们目前的研究表明，基于供体依赖性和用于培养和分化的培养基类型，人脐带血来源的肥大细胞（hCBMCs）的标志物表达存在显着差异。考虑到获得影响MC表型标志物表达的专用培养基的挑战，这些发现特别相关。结果发现，在StemSpanTM-XF培养液中培养的hCBMCs有中等水平的肥大/干细胞生长因子受体Kit（c-KIT）（mRNA和蛋白）表达，FcεRI（mRNA和蛋白）和TLR 2（mRNA和蛋白）表达较低，而MRGPRX 2（mRNA和蛋白）表达较高。相比之下，与StemSpanTM-XF培养基中培养的hCBMCs相比，Stem Line II培养基中培养的hCBMCs表达FcεRI和TLR 2（mRNA和蛋白质），c-KIT较高，但MRGPRX 2表达较低。这些结果表明，它是至关重要的，以考虑捐助者的依赖性和介质时，调查MC的功能，需要进一步的研究，以充分了解这些因素对hCBMC标志物表达的影响。
Mast cells (MCs) are tissue-resident immune cells of a hematopoietic origin that play vital roles in innate and adaptive immunity. Human MCs can be isolated and differentiated from various tissue sources, including cord blood, when supplemented with cytokines such as stem cell factor, interleukin 3, and interleukin 6. Our current research study has shown significant differences in the marker expressions of human cord blood-derived mast cells (hCBMCs) based on donor dependency and the type of medium used for culturing and differentiation. These findings are particularly relevant given the challenges of obtaining specialty media influencing MC phenotypic marker expressions. We found that hCBMCs cultured in StemSpanTM-XF medium had a moderate expression of mast/stem cell growth factor receptor Kit (c-KIT) (mRNA and protein), low expressions of FcεRI (mRNA) and TLR2 (mRNA and protein) but had high levels of MRGPRX2 (mRNA and protein) expressions. In contrast, hCBMCs cultured in Stem Line II medium expressed FcεRI and TLR2 (mRNA and protein) with higher c-KIT but had lower MRGPRX2 expressions compared to the hCBMCs cultured in the StemSpanTM-XF medium. These results suggest that it is crucial to consider both donor dependency and the medium when investigating MC functions and that further research is needed to fully understand the impact of these factors on the hCBMC marker expressions.
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影响因子：
39.3
作者：
Li D;Wu M
通讯作者：
Wu M
影响因子：
64.5
作者：
HUANG, E;NOCKA, K;BESMER, P
通讯作者：
BESMER, P
影响因子：
--
作者：
Kulka, Marianna;Metcalfe, Dean D
通讯作者：
Metcalfe, Dean D
DOI：
10.1073/pnas.180318697
发表时间：
2000-09-12
影响因子：
11.1
作者：
Ochi, H;De Jesus, NH;Boyce, JA
通讯作者：
Boyce, JA
影响因子：
20.3
作者：
RENNICK, D;HUNTE, B;THOMPSONSNIPES, L
通讯作者：
THOMPSONSNIPES, L