Lymphocyte/macrophage interactions: biomaterial surface-dependent cytokine, chemokine, and matrix protein production.
Lymphocyte/macrophage interactions: biomaterial surface-dependent cytokine, chemokine, and matrix protein production.
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DOI：
10.1002/jbm.a.31630
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发表时间：
2008-12-01
影响因子：
4.9
通讯作者：
Anderson, James M.
中科院分区：
文献类型：
--
作者：
Chang, David T.;Jones, Jacqueline A.;Meyerson, Howard;Colton, Erica;Kwon, Il Keun;Matsuda, Takehisa;Anderson, James M.
尽管淋巴细胞在生物材料植入部位短暂出现，但对淋巴细胞在合成聚合物生物反应中的作用知之甚少。为了研究淋巴细胞和巨噬细胞产生的细胞因子、趋化因子和细胞外基质(ECM)蛋白对生物材料表面的响应，将人外周血单核细胞和淋巴细胞共培养在聚对苯二甲酸乙二醇酯(PET)材料表面，表现出明显的疏水性、亲水性/中性、亲水性/阴离子和亲水性/阳离子化学性质。抗体阵列筛选显示，大多数检测到的蛋白质是炎症介质，指导伤口愈合的早期炎症阶段。分别于培养3、7、10d进行蛋白质组学、酶联免疫吸附测定和贴壁细胞分析。IL-2和干扰素-γ在任何共培养体系中均未检测到，提示缺乏淋巴细胞活化。相对于疏水的PET表面，亲水/中性表面增加了IL-8(p<；0.05)。与疏水表面相比，亲水/阴离子表面促进了肿瘤坏死因子-α在疏水和阳离子表面的增加，并增加了mip-1的β(p<；0.05)。由于在亲水性/阴离子表面观察到增强的巨噬细胞融合，这些细胞因子的产生可能在融合过程中起重要作用。与亲水/中性和阴离子表面相比，亲水/阳离子表面可促进IL-10的产生，并增加基质金属蛋白酶-9/基质金属蛋白酶组织抑制物(TIMP)(p<；0.05)。这些结果表明，亲水性/中性和阴离子表面促进了促炎反应，减少了ECM的降解，而亲水/阳离子表面诱导了抗炎反应，并增加了MMP-9/TIMP，增加了ECM破裂的可能性。这项研究还强调了蛋白质阵列在评估可溶性介质在生物材料炎症反应中的作用方面的有用性。
The role of lymphocytes in the biological response to synthetic polymers is poorly understood despite the transient appearance of lymphocytes at the biomaterial implant site. To investigate cytokines, chemokines, and extracellular matrix (ECM) proteins produced by lymphocytes and macrophages in response to biomaterial surfaces, human peripheral blood monocytes and lymphocytes were co-cultured on polyethylene terephthalate (PET)-based material surfaces displaying distinct hydrophobic, hydrophilic/neutral, hydrophilic/anionic, and hydrophilic/cationic chemistries. Antibody array screening showed the majority of detected proteins are inflammatory mediators that guide the early inflammatory phases of wound healing. Proteomic ELISA quantification and adherent cell analysis were performed after 3, 7, and 10 days of culture. IL-2 and IFN-γ were not detected in any co-cultures suggesting lack of lymphocyte activation. The hydrophilic/neutral surfaces increased IL-8 relative to the hydrophobic PET surface (p<0.05). The hydrophilic/anionic surfaces promoted increased TNF-α over hydrophobic and cationic surfaces and increased MIP-1β compared to hydrophobic surfaces (p<0.05). Since enhanced macrophage fusion was observed on hydrophilic/anionic surfaces, the production of these cytokines likely plays an important role in the fusion process. The hydrophilic/cationic surface promoted IL-10 production and increased matrix metalloproteinase (MMP)-9/tissue inhibitor of MMP (TIMP) relative to hydrophilic/neutral and anionic surfaces (p<0.05). These results suggest hydrophilic/neutral and anionic surfaces promote pro-inflammatory responses and reduced degradation of the ECM, whereas the hydrophilic/cationic surfaces induce an anti-inflammatory response and greater MMP-9/TIMP with an enhanced potential for ECM breakdown. The study also underscores the usefulness of protein arrays in assessing the role of soluble mediators in the inflammatory response to biomaterials.
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影响因子：
14
作者：
Bergman, AJ;Zygourakis, K
通讯作者：
Zygourakis, K
影响因子：
12.8
作者：
Groux, H;Cottrez, F
通讯作者：
Cottrez, F
DOI：
10.1002/jbm.820291014
发表时间：
1995-10-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
KAO, WYJ;MCNALLY, AK;ANDERSON, JM
通讯作者：
ANDERSON, JM
影响因子：
5.4
作者：
Maghazachi, AA;AlAoukaty, A;Schall, TJ
通讯作者：
Schall, TJ
DOI：
10.1002/jbm.820200104
发表时间：
1986-01-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
MAEDA, M;KIMURA, M;SAKURAI, Y
通讯作者：
SAKURAI, Y