Human gut microbiota: repertoire and variations.
Human gut microbiota: repertoire and variations.
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DOI：
10.3389/fcimb.2012.00136
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发表时间：
2012
影响因子：
5.7
通讯作者：
Raoult D
中科院分区：
文献类型：
--
作者：
Lagier JC;Million M;Hugon P;Armougom F;Raoult D
人类肠道微生物群的组成及其与宿主的关系，从而与人类健康和疾病的关系，对微生物学家提出了一些挑战。最初由依赖于文化的方法来探索这个生态系统，分子工具的出现彻底改变了我们调查这些关系的能力。然而，已经确定了许多导致矛盾结果的偏见。微生物培养组学是一个新的概念，它基于使用几种培养条件，通过MALDI-TOF进行鉴定，然后对培养的新物种进行基因组测序，这使得微生物培养组学与宏基因组学具有互补性。培养组学允许分离31个新的细菌物种，最大的人类病毒，最大的细菌，和最大的细菌从人类。此外，该生态系统中的一些成员，如真核生物、巨型病毒、浮游菌和浮游菌，被大多数研究所忽视。此外，许多因素，如年龄，地理来源，饮食习惯，抗生素或益生菌，可以影响微生物群的组成。最后，除了与研究技术相关的无数偏见外，对人类肠道微生物群研究的解释存在相当大的限制，这与资金来源和透明度披露有关。在未来，独立于食品工业资金的研究，并使用来自广泛的基于文化和分子工具的补充方法，将增加我们对这种复杂生态系统和宿主-微生物群互惠关系的了解。
The composition of human gut microbiota and their relationship with the host and, consequently, with human health and disease, presents several challenges to microbiologists. Originally dominated by culture-dependent methods for exploring this ecosystem, the advent of molecular tools has revolutionized our ability to investigate these relationships. However, many biases that have led to contradictory results have been identified. Microbial culturomics, a recent concept based on a use of several culture conditions with identification by MALDI-TOF followed by the genome sequencing of the new species cultured had allowed a complementarity with metagenomics. Culturomics allowed to isolate 31 new bacterial species, the largest human virus, the largest bacteria, and the largest Archaea from human. Moreover, some members of this ecosystem, such as Eukaryotes, giant viruses, Archaea, and Planctomycetes, have been neglected by the majority of studies. In addition, numerous factors, such as age, geographic provenance, dietary habits, antibiotics, or probiotics, can influence the composition of the microbiota. Finally, in addition to the countless biases associated with the study techniques, a considerable limitation to the interpretation of studies of human gut microbiota is associated with funding sources and transparency disclosures. In the future, studies independent of food industry funding and using complementary methods from a broad range of both culture-based and molecular tools will increase our knowledge of the repertoire of this complex ecosystem and host-microbiota mutualism.
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影响因子：
3.7
作者：
Armougom F;Henry M;Vialettes B;Raccah D;Raoult D
通讯作者：
Raoult D
影响因子：
3.7
作者：
Angelakis E;Raoult D
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影响因子：
3.6
作者：
Balamurugan, Ramadass;George, Gemlyn;Ramakrishna, Balakrishnan S.
通讯作者：
Ramakrishna, Balakrishnan S.
影响因子：
64.8
作者：
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通讯作者：
Bork, Peer
影响因子：
3.8
作者：
Angelakis, Emmanouil;Bastelica, Delphine;Raoult, Didier
通讯作者：
Raoult, Didier