Regenerative growth in Drosophila imaginal discs is regulated by Wingless and Myc.
Regenerative growth in Drosophila imaginal discs is regulated by Wingless and Myc.
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DOI：
10.1016/j.devcel.2009.04.015
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发表时间：
2009-06
影响因子：
11.8
通讯作者：
Hariharan, Iswar K.
中科院分区：
文献类型：
--
作者：
Smith-Bolton, Rachel K.;Worley, Melanie I.;Kanda, Hiroshi;Hariharan, Iswar K.
支持大规模遗传筛选的系统将极大地帮助再生研究。在这里，我们描述了一种非手术方法，通过以空间和时间调节的方式诱导翼成虫盘细胞凋亡来诱导果蝇幼虫的组织损伤和再生。组织损伤导致局部再生增殖，其特征是模式基因和生长调节剂的表达改变以及细胞命运承诺标记的暂时丧失。 Wingless 和 Myc 是由组织损伤诱导的，对于再生生长很重要。此外，异位 Myc 可以增强再生，而测试的其他生长驱动剂则不能。随着动物的成熟，再生能力会丧失，并且不能通过激活 Wg 或 Myc 来恢复。该系统有利于正向遗传筛选，能够公正地搜索调节再生程度和能力的基因。
The study of regeneration would be aided greatly by systems that support large-scale genetic screens. Here we describe a non-surgical method for inducing tissue damage and regeneration in Drosophila larvae by inducing apoptosis in the wing imaginal disc in a spatially and temporally regulated manner. Tissue damage results in localized regenerative proliferation characterized by altered expression of patterning genes and growth regulators as well as a temporary loss of markers of cell fate commitment. Wingless and Myc are induced by tissue damage and are important for regenerative growth. Furthermore, ectopic Myc enhances regeneration when other growth drivers tested do not. As the animal matures, the ability to regenerate is lost and cannot be restored by activation of Wg or Myc. This system is conducive to forward genetic screens, enabling an unbiased search for genes that regulate both the extent of and the capacity for regeneration.
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影响因子：
2.7
作者：
Bosch, M;Serras, F;Baguñà, J
通讯作者：
Baguñà, J
影响因子：
11.8
作者：
Beck, CW;Christen, B;Slack, JMW
通讯作者：
Slack, JMW
影响因子：
56.9
作者：
Gurley, Kyle A.;Rink, Jochen C.;Alvarado, Alejandro Sanchez
通讯作者：
Alvarado, Alejandro Sanchez
影响因子：
21.3
作者：
Johnston, LA;Sanders, AL
通讯作者：
Sanders, AL
影响因子：
56.9
作者：
HUSSEY, R. G.;THOMPSON, W. R.;CALHOUN, E. T.
通讯作者：
CALHOUN, E. T.