Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions.
Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions.
复制标题
DOI：
10.3389/fgene.2021.795706
复制
发表时间：
2021
影响因子：
3.7
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Chan J;Wang L;Li L;Mu K;Bushek D;Xu Y;Guo X;Zhang G;Zhang L
传染病的爆发正导致包括珊瑚、海星、虾和软体动物在内的海洋无脊椎动物数量普遍下降。“德尔莫病”（Dermo）是由原生生物海洋帕金虫（Perkinsus marinus）引起的，对东部牡蛎（Crassostrea virginica）具有致命性。太平洋牡蛎（Crassostrea gigas）对“德尔莫病”具有抗性，这是由于宿主与寄生虫之间的相互作用存在差异，但这种差异尚未得到充分理解。我们比较了这两种牡蛎在感染海洋帕金虫的早期和晚期的转录组反应。在这两个物种中，我们观察到大量先天免疫反应基因受到动态且有序的调控，大多数直系同源基因的调控模式极为相似，尽管东部牡蛎的反应更为强烈，这表明强烈或过度的免疫反应可能是宿主死亡的一个原因。在这两个物种之间，几个关键免疫反应基因家族在基因扩增、序列变异和/或对海洋帕金虫的转录反应方面存在差异，这反映了宿主 - 寄生虫相互作用的进化分歧。值得注意的是，在具有抗性的太平洋牡蛎中观察到凋亡抑制蛋白（IAPs）显著上调，而在易感的东部牡蛎中则没有，这表明IAPs的上调是一种主动防御机制，而非由海洋帕金虫主导的被动反应。与太平洋牡蛎相比，东部牡蛎的Toll样受体（TLRs）扩增更为显著，且在对海洋帕金虫有反应的TLRs中存在正选择。含有C1q结构域且带有参与识别海洋帕金虫的半乳糖结合凝集素结构域的蛋白质（C1qDCs）仅在东部牡蛎中存在且显著上调。这些结果揭示了这两种牡蛎在宿主防御基因方面此前未被描述的差异，这些差异可能解释了它们易感性的不同，为缺乏适应性免疫的冠轮动物中宿主 - 寄生虫相互作用的进化动态提供了更全面的描述。我们的研究结果表明，东部牡蛎和海洋帕金虫存在共同进化的历史，近期疾病的爆发可能是由于寄生虫毒力的增强。
Infectious disease outbreaks are causing widespread declines of marine invertebrates including corals, sea stars, shrimps, and molluscs. Dermo is a lethal infectious disease of the eastern oyster Crassostrea virginica caused by the protist Perkinsus marinus. The Pacific oyster Crassostrea gigas is resistant to Dermo due to differences in the host-parasite interaction that is not well understood. We compared transcriptomic responses to P. marinus challenge in the two oysters at early and late infection stages. Dynamic and orchestrated regulation of large sets of innate immune response genes were observed in both species with remarkably similar patterns for most orthologs, although responses in C. virginica were stronger, suggesting strong or over-reacting immune response could be a cause of host mortality. Between the two species, several key immune response gene families differed in their expansion, sequence variation and/or transcriptional response to P. marinus, reflecting evolutionary divergence in host-parasite interaction. Of note, significant upregulation of inhibitors of apoptosis (IAPs) was observed in resistant C. gigas but not in susceptible C. virginica, suggesting upregulation of IAPs is an active defense mechanism, not a passive response orchestrated by P. marinus. Compared with C. gigas, C. virginica exhibited greater expansion of toll-like receptors (TLRs) and positive selection in P. marinus responsive TLRs. The C1q domain containing proteins (C1qDCs) with the galactose-binding lectin domain that is involved in P. marinus recognition, were only present and significantly upregulated in C. virginica. These results point to previously undescribed differences in host defense genes between the two oyster species that may account for the difference in susceptibility, providing an expanded portrait of the evolutionary dynamics of host-parasite interaction in lophotrochozoans that lack adaptive immunity. Our findings suggest that C. virginica and P. marinus have a history of coevolution and the recent outbreaks may be due to increased virulence of the parasite.
登录
查看更多内容
影响因子：
4.6
作者：
Carnegie RB;Ford SE;Crockett RK;Kingsley-Smith PR;Bienlien LM;Safi LSL;Whitefleet-Smith LA;Burreson EM
通讯作者：
Burreson EM
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
2.4
作者：
Bidegain, G.;Powell, E. N.;Guo, X.
通讯作者：
Guo, X.
影响因子：
10.7
作者：
Alcaide, Miguel;Edwards, Scott V.
通讯作者：
Edwards, Scott V.
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T