Airway branching has conserved needs for local parasympathetic innervation but not neurotransmission.
Airway branching has conserved needs for local parasympathetic innervation but not neurotransmission.
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DOI：
10.1186/s12915-014-0092-2
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发表时间：
2014-11-11
期刊：
影响因子：
5.4
通讯作者：
Jesudason EC
中科院分区：
文献类型：
--
作者：
Bower DV;Lee HK;Lansford R;Zinn K;Warburton D;Fraser SE;Jesudason EC
副交感神经信号已被推测为调节上皮分支以及器官再生和肿瘤发展。然而，局部神经接触与分泌信号的相对贡献仍不清楚。在这里，我们显示了一种保守的(脊椎动物到无脊椎动物)对呼吸道分支中完整的局部神经的要求，即使胆碱能神经传递被阻断，这种要求仍然存在。在脊椎动物的肺中，使用双光子激光删除增强型绿色荧光蛋白(EGFP)标记的内在神经元，会使相邻细胞保持完好，但会取消分支。分支不受邻近非神经中胚层组织的相似激光功率的影响，通过阻断胆碱能受体或肉毒杆菌毒素A阻断突触传递。由于邻近的血管和上皮细胞的增殖也有助于脊椎动物肺的分支，因此通过删除果蝇胚胎中的神经元来测试对神经的直接依赖来实现呼吸道分支。通过在泛神经元ELAV增强子下驱动细胞自主的蓖麻毒素A来特异性缺失果蝇胚胎中的神经元，扰乱了果蝇的呼吸道发育。该系统证实，即使在没有血管系统或上皮细胞增殖的情况下，气道分支仍然受到神经损害的干扰。综上所述，这表明在脊椎动物和无脊椎动物中，呼吸道形态发生需要局部神经支配，而神经传递是必不可少的。苍蝇对神经支配的需求持续存在，而邻近的血管系统和上皮细胞增殖则不存在。我们的新型靶向激光消融技术使副交感神经支配的局部功能与神经传递区分开来。本文的在线版本(doi：10.1186/s12915-0140092-2)包含补充材料，授权用户可以使用。
Parasympathetic signaling has been inferred to regulate epithelial branching as well as organ regeneration and tumor development. However, the relative contribution of local nerve contact versus secreted signals remains unclear. Here, we show a conserved (vertebrates to invertebrates) requirement for intact local nerves in airway branching, persisting even when cholinergic neurotransmission is blocked. In the vertebrate lung, deleting enhanced green fluorescent protein (eGFP)-labeled intrinsic neurons using a two-photon laser leaves adjacent cells intact, but abolishes branching. Branching is unaffected by similar laser power delivered to the immediately adjacent non-neural mesodermal tissue, by blocking cholinergic receptors or by blocking synaptic transmission with botulinum toxin A. Because adjacent vasculature and epithelial proliferation also contribute to branching in the vertebrate lung, the direct dependence on nerves for airway branching was tested by deleting neurons in Drosophila embryos. A specific deletion of neurons in the Drosophila embryo by driving cell-autonomous RicinA under the pan-neuronal elav enhancer perturbed Drosophila airway development. This system confirmed that even in the absence of a vasculature or epithelial proliferation, airway branching is still disrupted by neural lesioning. Together, this shows that airway morphogenesis requires local innervation in vertebrates and invertebrates, yet neurotransmission is dispensable. The need for innervation persists in the fly, wherein adjacent vasculature and epithelial proliferation are absent. Our novel, targeted laser ablation technique permitted the local function of parasympathetic innervation to be distinguished from neurotransmission. The online version of this article (doi:10.1186/s12915-014-0092-2) contains supplementary material, which is available to authorized users.
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影响因子：
56.9
作者：
Lammert, E;Cleaver, O;Melton, D
通讯作者：
Melton, D
影响因子：
2.4
作者：
Freem, Lucy J.;Escot, Sophie;Burns, Alan J.
通讯作者：
Burns, Alan J.
平铺 3D 显微图像采集的全局最佳拼接。
DOI：
10.1093/bioinformatics/btp184
发表时间：
2009-06-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Preibisch S;Saalfeld S;Tomancak P
通讯作者：
Tomancak P
影响因子：
64.5
作者：
Sutherland, D;Samakovlis, C;Krasnow, MA
通讯作者：
Krasnow, MA
影响因子：
--
作者：
Warburton, David;El-Hashash, Ahmed;Carraro, Gianni;Tiozzo, Caterina;Sala, Frederic;Rogers, Orquidea;De Langhe, Stijn;Kemp, Paul J.;Riccardi, Daniela;Torday, John;Bellusci, Saverio;Shi, Wei;Lubkin, Sharon R.;Jesudason, Edwin
通讯作者：
Jesudason, Edwin