Biotransformation of the mycotoxin deoxynivalenol in fusarium resistant and susceptible near isogenic wheat lines.
Biotransformation of the mycotoxin deoxynivalenol in fusarium resistant and susceptible near isogenic wheat lines.
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DOI：
10.1371/journal.pone.0119656
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Schuhmacher R
中科院分区：
文献类型：
--
作者：
Kluger B;Bueschl C;Lemmens M;Michlmayr H;Malachova A;Koutnik A;Maloku I;Berthiller F;Adam G;Krska R;Schuhmacher R
本研究采用液相色谱-高分辨率质谱联用技术对小麦镰刀菌毒素脱氧雪腐镰刀菌毒素（DON）解毒过程中形成的9种不同生物转化产物进行了表征。检测到的代谢物表明，DON通过两种主要的代谢途径与内源性代谢物结合，即1)糖基化（DON-3-葡萄糖苷、DON-二己糖、15-乙酰-DON-3-葡萄糖苷、DON-丙二酰葡萄糖苷）和2)谷胱甘肽结合（DON- s -谷胱甘肽、“DON- 2h”- s -谷胱甘肽、DON- s -半胱氨酸甘氨酸和DON- s -半胱氨酸）。此外，还发现DON与一种假定的糖醇（己醇）偶联。用1 mg DON处理的“Remus”品种的摩尔质量平衡显示，在测试条件下，大约15%的添加DON转化为DON-3-葡萄糖苷，另外19%转化为其余8种生物转化产物或不可逆地结合到植物基质上。此外，还监测了每种DON衍生物的代谢物丰度随时间的变化，并建立了6个DON处理小麦品系（1mg /穗）的抗性数量性状位点（QTL） Fhb1和/或Qfhs.ifa-5A。所有携带Fhb1 QTL的品种均表现出相似的代谢动力学：与缺乏Fhb1抗性QTL的品种相比，DON-Glc的形成更快，而DON-GSH的产生效率更低。此外，所有携带Fhb1的小麦品系的D3G/DON丰度比均显著升高。
In this study, a total of nine different biotransformation products of the Fusarium mycotoxin deoxynivalenol (DON) formed in wheat during detoxification of the toxin are characterized by liquid chromatography—high resolution mass spectrometry (LC-HRMS). The detected metabolites suggest that DON is conjugated to endogenous metabolites via two major metabolism routes, namely 1) glucosylation (DON-3-glucoside, DON-di-hexoside, 15-acetyl-DON-3-glucoside, DON-malonylglucoside) and 2) glutathione conjugation (DON-S-glutathione, “DON-2H”-S-glutathione, DON-S-cysteinyl-glycine and DON-S-cysteine). Furthermore, conjugation of DON to a putative sugar alcohol (hexitol) was found. A molar mass balance for the cultivar ‘Remus’ treated with 1 mg DON revealed that under the test conditions approximately 15% of the added DON were transformed into DON-3-glucoside and another 19% were transformed to the remaining eight biotransformation products or irreversibly bound to the plant matrix. Additionally, metabolite abundance was monitored as a function of time for each DON derivative and was established for six DON treated wheat lines (1 mg/ear) differing in resistance quantitative trait loci (QTL) Fhb1 and/or Qfhs.ifa-5A. All cultivars carrying QTL Fhb1 showed similar metabolism kinetics: Formation of DON-Glc was faster, while DON-GSH production was less efficient compared to cultivars which lacked the resistance QTL Fhb1. Moreover, all wheat lines harboring Fhb1 showed significantly elevated D3G/DON abundance ratios.
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影响因子：
3.5
作者：
Berthiller F;Krska R;Domig KJ;Kneifel W;Juge N;Schuhmacher R;Adam G
通讯作者：
Adam G
影响因子：
5.4
作者：
Buerstmayr, H;Lemmens, M;Ruckenbauer, P
通讯作者：
Ruckenbauer, P
影响因子：
3
作者：
Dall'asta, C;Berthiller, F;Krska, R
通讯作者：
Krska, R
影响因子：
5.4
作者：
Anderson, JA;Stack, RW;Frohberg, RC
通讯作者：
Frohberg, RC
影响因子：
4.2
作者：
Fruhmann P;Hametner C;Mikula H;Adam G;Krska R;Fröhlich J
通讯作者：
Fröhlich J