Transcriptomics-Based Identification of Genes Related to Tapetum Degradation and Microspore Development in Lily.
Transcriptomics-Based Identification of Genes Related to Tapetum Degradation and Microspore Development in Lily.
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基于转录组学的百合绒毡层降解和小孢子发育相关基因的鉴定
DOI：
10.3390/genes13020366
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发表时间：
2022-02-17
期刊：
影响因子：
3.5
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Sui J;Jia W;Xin Y;Zhang Y
百合是一种世界性的观赏经济作物。然而，其花粉粒的高产量给消费者带来了严重的问题，包括过敏和衣服染色。在花药发育过程中，绒毡层是花粉形成和小孢子释放的关键步骤。因此，研究百合游离花粉污染环境下绒毡层降解和小孢子发育的机理具有重要意义。在这里，我们使用切割百合品种'西伯利亚'的特点绒毡层降解的过程中，通过使用细胞学和转录组学的方法。细胞学观察表明，随着百合芽从4cm（Lo 4cm）发育到8 cm（Lo 8 cm），绒毡层完成降解过程，小孢子成熟。此外，通过比较三个发育阶段（Lo 4 cm，Lo 6 cm和Lo 8 cm）的转录组谱，我们确定了27个差异表达的基因。根据功能将这27个基因分为4类，即细胞分裂和扩增、细胞壁形态发生、转录因子、LRR-RLK（leucine-rich repeat receptor like kinases）、植物激素合成和转导。进行定量实时PCR作为转录组数据的验证。这些基因是控制百合绒毡层退化和小孢子发育的候选基因，为培育无花粉百合新品种提供了理论依据。
Lily is a popular and economically ornamental crop around the world. However, its high production of pollen grains causes serious problems to consumers, including allergies and staining of clothes. During anther development, the tapetum is a crucial step for pollen formation and microspore release. Therefore, it is important to understand the mechanism of tapetum degradation and microspore development in lily where free pollen contamination occurs. Here, we used the cut lily cultivar ‘Siberia’ to characterize the process of tapetum degradation through the use of cytology and transcriptomic methods. The cytological observation indicated that, as the lily buds developed from 4 cm (Lo 4 cm) to 8 cm (Lo 8 cm), the tapetum completed the degradation process and the microspores matured. Furthermore, by comparing the transcriptome profiling among three developmental stages (Lo 4 cm, Lo 6 cm and Lo 8 cm), we identified 27 differentially expressed genes. These 27 genes were classed into 4 groups by function, namely, cell division and expansion, cell-wall morphogenesis, transcription factors, LRR-RLK (leucine-rich repeat receptor-like kinases), plant hormone biosynthesis and transduction. Quantitative real-time PCR was performed as validation of the transcriptome data. These selected genes are candidate genes for the tapetum degradation and microspore development of lily and our work provides a theoretical basis for breeding new lily cultivars without pollen.
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通过转录组和蛋白质组数据分析百合花粉过敏原
影响因子：
5.6
作者：
Feng, Jingxian;Wu, Ze;Teng, Nianjun
通讯作者：
Teng, Nianjun
DOI：
10.1073/pnas.2018565117
发表时间：
2020-12-22
影响因子：
11.1
作者：
Crook AD;Willoughby AC;Hazak O;Okuda S;VanDerMolen KR;Soyars CL;Cattaneo P;Clark NM;Sozzani R;Hothorn M;Hardtke CS;Nimchuk ZL
通讯作者：
Nimchuk ZL
DOI：
10.1093/bioinformatics/bts565
发表时间：
2012-12-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
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作者：
Fu L;Niu B;Zhu Z;Wu S;Li W
通讯作者：
Li W
影响因子：
5.6
作者：
Guo J;Liu C;Wang P;Cheng Q;Sun L;Yang W;Shen H
通讯作者：
Shen H
影响因子：
14.9
作者：
Harris, MA;Clark, J;White, R
通讯作者：
White, R