Single-molecule and particle detection on true portable microscopy platforms

Single-molecule and particle detection on true portable microscopy platforms
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在真正的便携式显微镜平台上进行单分子和颗粒检测

DOI:
10.1016/j.snr.2021.100063
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发表时间:
2022
影响因子:
5.9
通讯作者:
Wei, Qingshan
Wei, Qingshan
中科院分区:
--
文献类型:
--
作者:
Skolrood, Lydia;Wang, Yan;Zhang, Shengwei;Wei, Qingshan

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能够进行早期疾病检测和治疗监测的护理点技术(POCT)对于下一代诊断和个性化医疗至关重要。与此同时,全球需要低成本的POCT,使资源有限的设置能够获得先进的诊断工具。近年来,多个用于单分子和颗粒检测的移动成像平台相继问世,极大地提高了分子检测的检测灵敏度。这篇综述重点介绍了在真正的便携式平台上实现单分子和粒子光学检测的新兴技术。讨论了基于智能手机、单板计算机(即,树莓PI系统)、单芯片实验室系统和3D打印显微镜平台的微型、高灵敏度成像设备。
Point-of-care technologies (POCT) that enable early disease detection and therapeutic monitoring are crucial for the next generation of diagnostics and personalized medicine. Meanwhile, there is a global need for low-cost POCT that makes advanced diagnostic tools accessible to resource-limited settings. Recently, several mobile imaging platforms for single-molecule and particle detection have been developed, which greatly improve the detection sensitivity of molecular assays. This review highlights emerging technologies that achieve single-molecule and particle optical detection on true portable platforms. Miniature, high-sensitivity imaging devices based on smartphones, single-board computers (i.e., Raspberry Pi systems), lab-on-a-chip systems, and 3D-printed microscopy platforms are discussed.
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