Computational sensing of herpes simplex virus using a cost-effective on-chip microscope.
Computational sensing of herpes simplex virus using a cost-effective on-chip microscope.
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DOI：
10.1038/s41598-017-05124-3
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发表时间：
2017-07-07
影响因子：
4.6
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Ray A;Daloglu MU;Ho J;Torres A;Mcleod E;Ozcan A
单纯疱疹病毒（HSV）是由单纯疱疹病毒（HSV）引起的，是一种病毒感染，是世界范围内最广泛的疾病之一。在这里，我们提出了一种计算传感技术，用于使用病毒免疫特异性和病毒的物理大小范围来特异性检测HSV。这种无标记的方法涉及一个紧凑和具有成本效益的全息片上显微镜和表面功能化的玻璃基板准备专门捕捉目标病毒。为了增强单个病毒的光学特征并提高其信噪比，使用便携式接口在基底上捕获的每个病毒颗粒周围快速形成基于自组装聚乙二醇的纳米透镜。然后重建被这些自组装纳米透镜包围的特定捕获病毒的全息阴影，并将相位图像用于自动量化我们的大视场（约30 mm 2）内每个颗粒的大小。由于表面功能化和全息成像实现的物理尺寸测量的病毒免疫特异性的组合用于使用我们紧凑且具有成本效益的平台灵敏地检测和计数HSV颗粒。这种计算感测技术可以在资源有限的环境中的全球健康相关应用中找到许多用途。
Caused by the herpes simplex virus (HSV), herpes is a viral infection that is one of the most widespread diseases worldwide. Here we present a computational sensing technique for specific detection of HSV using both viral immuno-specificity and the physical size range of the viruses. This label-free approach involves a compact and cost-effective holographic on-chip microscope and a surface-functionalized glass substrate prepared to specifically capture the target viruses. To enhance the optical signatures of individual viruses and increase their signal-to-noise ratio, self-assembled polyethylene glycol based nanolenses are rapidly formed around each virus particle captured on the substrate using a portable interface. Holographic shadows of specifically captured viruses that are surrounded by these self-assembled nanolenses are then reconstructed, and the phase image is used for automated quantification of the size of each particle within our large field-of-view, ~30 mm2. The combination of viral immuno-specificity due to surface functionalization and the physical size measurements enabled by holographic imaging is used to sensitively detect and enumerate HSV particles using our compact and cost-effective platform. This computational sensing technique can find numerous uses in global health related applications in resource-limited environments.
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影响因子：
17.1
作者：
McLeod, Euan;Chau Nguyen;Huang, Patrick;Luo, Wei;Veli, Muhammed;Ozcan, Aydogan
通讯作者：
Ozcan, Aydogan
影响因子：
6.1
作者：
Bishara W;Sikora U;Mudanyali O;Su TW;Yaglidere O;Luckhart S;Ozcan A
通讯作者：
Ozcan A
影响因子：
3.7
作者：
Greenbaum A;Akbari N;Feizi A;Luo W;Ozcan A
通讯作者：
Ozcan A
影响因子：
6.4
作者：
Bradley, Heather;Markowitz, Lauri E.;McQuillan, Geraldine M.
通讯作者：
McQuillan, Geraldine M.
影响因子：
3.8
作者：
Bishara W;Su TW;Coskun AF;Ozcan A
通讯作者：
Ozcan A