Identification and Characterization of an Arabidopsis thaliana Mutant lbt With High Tolerance to Boron Deficiency.
Identification and Characterization of an Arabidopsis thaliana Mutant lbt With High Tolerance to Boron Deficiency.
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缺硼高耐受性拟南芥突变体 lbt 的鉴定和表征
DOI：
10.3389/fpls.2018.00736
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发表时间：
2018
影响因子：
5.6
通讯作者：
Xu F
中科院分区：
文献类型：
--
作者：
Huai Z;Peng L;Wang S;Zhao H;Shi L;Xu F
硼（B）是植物必需的微量营养元素。在本研究中，我们的特点是拟南芥突变体lbt具有显着的低硼耐受性，确定了我们以前的定位的基础上，在拟南芥中的B效率的QTL。多种营养缺乏分析指出，lbt突变体是不敏感的，只有硼限制压力。与野生型Col-0相比，B缺乏显著提高了lbt突变体的鲜重、叶面积、根长和根伸长速率。在B限制下，lbt突变体在生殖生长期的株高、分枝数和花序数也比Col-0有明显的提高。叶片超微结构分析表明，lbt突变体的淀粉积累量明显低于Col-0。此外，在正常B和低B条件下，Col-0和lbt突变体之间的转运蛋白相关基因的表达和B浓度没有显着差异。这些结果表明lbt突变体比Col-0具有更低的B需求。遗传分析表明，lbt突变体的耐低硼表型受一对隐性单基因控制。基于高密度SNP连锁遗传图谱，仅将一个耐低硼QTL定位在第4染色体10.4 - 14.8 Mb之间。在该QTL区间内未发现B相关基因，表明lbt对B限制的耐受性受一个功能未知的基因控制。因此，lbt是一种不依赖于B吸收或转运的低B耐性突变体，受定位于第4染色体上的单基因隐性基因控制，LBT基因的克隆和功能分析有望揭示植物抗B缺乏的新机制。
Boron (B) is an essential micronutrient of plants. In the present study, we characterized an Arabidopsis mutant lbt with significant low-boron tolerance that was identified based on our previous mapping of QTL for B efficiency in Arabidopsis. Multiple nutrient-deficiency analyses point out that lbt mutant is insensitive to only B-limitation stress. Compared with wild-type Col-0, the fresh weight, leaf area, root length and root elongation rate of lbt mutant were significantly improved under B deficiency during vegetative growth. lbt mutant also showed the improvements in plant height, branches and inflorescences compared with Col-0 during the reproductive stage under B limitation. Ultrastructure analysis of the leaves showed that starch accumulation in lbt mutant was significantly diminished compared with Col-0. Furthermore, there were no significant differences in the expression of transporter-related genes and B concentrations between Col-0 and lbt mutant under both normal B and low-B conditions. These results suggest that lbt mutant has a lower B demand than Col-0. Genetic analysis suggests that the low-B tolerant phenotype of lbt mutant is under the control of a monogenic recessive gene. Based on the high-density SNP linkage genetic map, only one QTL for low-B tolerance was mapped on chromosome 4 between 10.4 and 14.8 Mb. No any reported B-relative genes exist in the QTL interval, suggesting that a gene with unknown function controls the tolerance of lbt to B limitation. Taken together, lbt is a low-B tolerant mutant that does not depend on the uptake or transport of B and is controlled by a monogenic recessive gene mapped on chromosome 4, and cloning and functional analysis of LBT gene are expected to reveal novel mechanisms for plant resistance to B deficiency.
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影响因子：
4.4
作者：
Guo H;Liu Y;Li X;Yan Z;Xie Y;Xiong H;Zhao L;Gu J;Zhao S;Liu L
通讯作者：
Liu L
影响因子：
7.4
作者：
FUJIWARA, T;HIRAI, MY;NAITO, S
通讯作者：
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DOI：
10.5560/znc.2012.67c0510
发表时间：
2012-09-01
影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
6.9
作者：
Camacho-Cristóbal JJ;Martín-Rejano EM;Herrera-Rodríguez MB;Navarro-Gochicoa MT;Rexach J;González-Fontes A
通讯作者：
González-Fontes A