Transient frictional slip between integrin and the ECM in focal adhesions under myosin II tension.
Transient frictional slip between integrin and the ECM in focal adhesions under myosin II tension.
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DOI：
10.1016/j.cub.2010.05.049
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发表时间：
2010-07-13
期刊：
影响因子：
9.2
通讯作者：
Gardel, Margaret L.
中科院分区：
文献类型：
--
作者：
Aratyn-Schaus, Yvonne;Gardel, Margaret L.
The spatiotemporal regulation of adhesion to the extracellular matrix is important in metazoan cell migration and mechanosensation. While adhesion assembly depends on intracellular and extracellular tension, the biophysical regulation of force transmission between the actin cytoskeleton and extracellular matrix during this process remains largely unknown. To elucidate the nature of force transmission as myosin-II tension is applied to focal adhesions, we correlated the dynamics of focal adhesion proteins and the actin cytoskeleton to local traction stresses. We find that, under low extracellular tension, newly formed adhesions near the cell periphery undergo a transient retrograde displacement preceding elongation. We identify that myosin-II generated tension drives this mobility and determine the interface of differential motion, or ‘slip’, to be between the integrin and the ECM. We found that the magnitude and duration of both adhesion slip and associated F-actin dynamics is strongly modulated by the ECM compliance. Furthermore, traction forces are generated throughout the slip period with adhesion immobilization occurring at a constant tension. We have identified a tension-dependent, extracellular ‘clutch’ between integrins and the extracellular matrix that is utilized to stabilize adhesions under myosin-II driven tension. This work signifies that modulating adhesion alters force transmission during focal adhesion maturation.
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影响因子：
3.2
作者：
Macdonald, Alice;Horwitz, A. Rick;Lauffenburger, Douglas A.
通讯作者：
Lauffenburger, Douglas A.
影响因子：
64.5
作者：
Choquet, D;Felsenfeld, DP;Sheetz, MP
通讯作者：
Sheetz, MP
DOI：
10.1083/jcb.200810002
发表时间：
2009-06-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kong F;García AJ;Mould AP;Humphries MJ;Zhu C
通讯作者：
Zhu C
影响因子：
7.8
作者：
Gardel, Margaret L.;Sabass, Benedikt;Ji, Lin;Danuser, Gaudenz;Schwarz, Ulrich S.;Waterman, Clare M.
通讯作者：
Waterman, Clare M.
影响因子：
56.9
作者：
Hu, Ke;Ji, Lin;Waterman-Storer, Clare M.
通讯作者：
Waterman-Storer, Clare M.