Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis.
Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis.
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DOI：
10.1186/1472-6831-11-33
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发表时间：
2011-12-12
期刊：
影响因子：
2.9
通讯作者：
Xu J
中科院分区：
文献类型：
--
作者：
Huang S;Yang F;Zeng X;Chen J;Li R;Wen T;Li C;Wei W;Liu J;Chen L;Davis C;Xu J
居住在人类口腔中的微生物群落与口腔健康和疾病有关。以往的研究表明，中国成人牙龈炎的普遍患病率较高。本研究的目的是通过高度平行焦磷酸测序确定微生物系统发育多样性和群落结构，深入表征患有或不患有牙龈炎的中国成年人的口腔微生物群。本横断面研究纳入了6例不吸烟的中国人，3例有牙龈炎，3例无牙龈炎，年龄21-39岁，女性4例，男性2例。临床医生使用Mazza牙龈指数（MGI）对探诊时炎症和出血的牙龈参数进行表征。每个受试者的牙菌斑（分别从四个不同的口腔部位取样）和唾液样本。通过焦磷酸测序确定细菌16s rDNA pcr扩增子的序列和相对丰度，产生400 bp长的reads。序列数据通过为人类口腔微生物组分析定制的计算管道进行分析。此外，采用定量PCR验证了所选微生物群的相对丰度。牙龈炎和健康受试者的口腔微生物组可以根据菌斑微生物组的不同群落结构进行区分，但不能根据唾液微生物组进行区分。群落成员对群落结构差异的贡献在门、属和类种水平上进行了统计分析。发现与牙龈炎相关的8个主要分类群：TM7、细毛菌、硒单胞菌、链球菌、细毛菌、普雷沃菌、嗜Lautropia和嗜血杆菌。此外，鉴定出98个物种水平的OTUs与牙龈炎相关，这在物种分辨率上提供了牙龈炎的微生物特征。最后，对于所选的链球菌和梭杆菌两个属，基于Real-Time PCR的相对细菌丰度定量验证了基于焦磷酸测序的结果。本方法的研究表明，该牙龈炎患者的口腔样本可以通过对16个S rDNA基因进行焦磷酸测序，通过菌斑微生物组来表征。进一步的研究，从更大的人群规模的受试者（纵向研究设计）中提取系列样本，可能会为临床确定的牙龈炎状态下口腔微生物群落的时间和生态特征提供见解。
Microbial communities inhabiting human mouth are associated with oral health and disease. Previous studies have indicated the general prevalence of adult gingivitis in China to be high. The aim of this study was to characterize in depth the oral microbiota of Chinese adults with or without gingivitis, by defining the microbial phylogenetic diversity and community-structure using highly paralleled pyrosequencing. Six non-smoking Chinese, three with and three without gingivitis (age range 21-39 years, 4 females and 2 males) were enrolled in the present cross-sectional study. Gingival parameters of inflammation and bleeding on probing were characterized by a clinician using the Mazza Gingival Index (MGI). Plaque (sampled separately from four different oral sites) and salivary samples were obtained from each subject. Sequences and relative abundance of the bacterial 16 S rDNA PCR-amplicons were determined via pyrosequencing that produced 400 bp-long reads. The sequence data were analyzed via a computational pipeline customized for human oral microbiome analyses. Furthermore, the relative abundances of selected microbial groups were validated using quantitative PCR. The oral microbiomes from gingivitis and healthy subjects could be distinguished based on the distinct community structures of plaque microbiomes, but not the salivary microbiomes. Contributions of community members to community structure divergence were statistically accessed at the phylum, genus and species-like levels. Eight predominant taxa were found associated with gingivitis: TM7, Leptotrichia, Selenomonas, Streptococcus, Veillonella, Prevotella, Lautropia, and Haemophilus. Furthermore, 98 species-level OTUs were identified to be gingivitis-associated, which provided microbial features of gingivitis at a species resolution. Finally, for the two selected genera Streptococcus and Fusobacterium, Real-Time PCR based quantification of relative bacterial abundance validated the pyrosequencing-based results. This methods study suggests that oral samples from this patient population of gingivitis can be characterized via plaque microbiome by pyrosequencing the 16 S rDNA genes. Further studies that characterize serial samples from subjects (longitudinal study design) with a larger population size may provide insight into the temporal and ecological features of oral microbial communities in clinically-defined states of gingivitis.
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影响因子：
4.4
作者：
Ashelford, KE;Chuzhanova, NA;Weightman, AJ
通讯作者：
Weightman, AJ
影响因子：
--
作者：
KONONEN, E;ASIKAINEN, S;JOUSIMIESSOMER, H
通讯作者：
JOUSIMIESSOMER, H
DOI：
10.1038/ismej.2010.30
发表时间：
2010-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.7
作者：
Griffen AL;Beall CJ;Firestone ND;Gross EL;Difranco JM;Hardman JH;Vriesendorp B;Faust RA;Janies DA;Leys EJ
通讯作者：
Leys EJ
影响因子：
2.7
作者：
Crielaard W;Zaura E;Schuller AA;Huse SM;Montijn RC;Keijser BJ
通讯作者：
Keijser BJ