Exploring the oral microbiota of children at various developmental stages of their dentition in the relation to their oral health.
Exploring the oral microbiota of children at various developmental stages of their dentition in the relation to their oral health.
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DOI：
10.1186/1755-8794-4-22
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发表时间：
2011-03-04
影响因子：
2.7
通讯作者：
Keijser BJ
中科院分区：
文献类型：
--
作者：
Crielaard W;Zaura E;Schuller AA;Huse SM;Montijn RC;Keijser BJ
了解共生菌群与健康的关系对预防疾病至关重要。本文研究了74名年龄在3 - 18岁的儿童从乳牙牙列自然过渡到恒牙列的口腔微生物组成，并将微生物特征与他们的口腔健康状况联系起来。采用16s rRNA的V5-V6高变区条形码焦磷酸测序和系统发育微阵列技术对唾液的微生物组成进行了评估。焦磷酸测序（126174条，1045条独特序列）共代表8个门和113个高级分类群。厚壁菌门、细菌门、变形菌门和放线菌门在各类群中均占主导地位。乳牙列中变形菌门（Gammaproteobacteria, Moraxellaceae）的比例高于拟杆菌门（Bacteroidetes），而在所有其他类群中，拟杆菌门的数量至少与变形菌门一样多。拟杆菌门（主要为Prevotella属）、细孢杆菌科、螺旋体和候选分支TM7随着年龄的增长而增加，反映了随着年龄的变化，微生物组在生物学变化的驱动下趋于成熟。微阵列分析可以进一步分析个体唾液微生物群。在350个微阵列探针中，156个给出了阳性信号，平均每个样品77个（范围48-93）探针。口腔无龋状态与靶向卡顿卟啉单胞菌和黄奈瑟菌的探针的高信号显著相关。catoniae和N. flavescens作为口腔健康标志物的潜在作用应在大规模的临床研究中进行评估。开放式和靶向分子方法的结合为我们提供了信息，这将增加我们对人类宿主与其微生物组之间相互作用的理解。
An understanding of the relation of commensal microbiota to health is essential in preventing disease. Here we studied the oral microbial composition of children (N = 74, aged 3 - 18 years) in natural transition from their deciduous to a permanent dentition and related the microbial profiles to their oral health status. The microbial composition of saliva was assessed by barcoded pyrosequencing of the V5-V6 hypervariable regions of the 16 S rRNA, as well as by using phylogenetic microarrays. Pyrosequencing reads (126174 reads, 1045 unique sequences) represented 8 phyla and 113 higher taxa in saliva samples. Four phyla - Firmicutes, Bacteriodetes, Proteobacteria and Actinobacteria - predominated in all groups. The deciduous dentition harboured a higher proportion of Proteobacteria (Gammaproteobacteria, Moraxellaceae) than Bacteroidetes, while in all other groups Bacteroidetes were at least as abundant as Proteobacteria. Bacteroidetes (mainly genus Prevotella), Veillonellaceae family, Spirochaetes and candidate division TM7 increased with increasing age, reflecting maturation of the microbiome driven by biological changes with age. Microarray analysis enabled further analysis of the individual salivary microbiota. Of 350 microarray probes, 156 gave a positive signal with, on average, 77 (range 48-93) probes per individual sample. A caries-free oral status significantly associated with the higher signal of the probes targeting Porphyromonas catoniae and Neisseria flavescens. The potential role of P. catoniae and N. flavescens as oral health markers should be assessed in large-scale clinical studies. The combination of both, open-ended and targeted molecular approaches provides us with information that will increase our understanding of the interplay between the human host and its microbiome.
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影响因子：
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作者：
Papaioannou, W.;Gizani, S.;Papagiannoulis, L.
通讯作者：
Papagiannoulis, L.
影响因子：
14.9
作者：
Ludwig, W;Strunk, O;Schleifer, KH
通讯作者：
Schleifer, KH
影响因子：
7.6
作者：
Könönen, E;Kanervo, A;Jousimies-Somer, H
通讯作者：
Jousimies-Somer, H
DOI：
10.14219/jada.archive.1964.0034
发表时间：
1964-01-01
影响因子：
3.9
作者：
GREENE, JC;VERMILLION, JR
通讯作者：
VERMILLION, JR
影响因子：
--
作者：
KONONEN, E;ASIKAINEN, S;JOUSIMIESSOMER, H
通讯作者：
JOUSIMIESSOMER, H