Fractional anisotropy from diffusion tensor imaging correlates with acute astrocyte and myelin swelling in neonatal swine models of excitotoxic and hypoxic-ischemic brain injury.
Fractional anisotropy from diffusion tensor imaging correlates with acute astrocyte and myelin swelling in neonatal swine models of excitotoxic and hypoxic-ischemic brain injury.
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DOI：
10.1002/cne.25121
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发表时间：
2021-07-01
期刊：
影响因子：
--
通讯作者：
Martin LJ
中科院分区：
文献类型：
--
作者：
Lee JK;Liu D;Jiang D;Kulikowicz E;Tekes A;Liu P;Qin Q;Koehler RC;Aggarwal M;Zhang J;Martin LJ
引起新生儿缺氧缺血（HI）后弥散张量成像（DTI）的各向异性分数（FA）和平均弥散度（MD）异常的特定细胞病理学尚未完全了解。大脑中细胞类型的多样性可能对DTI指标的变化有不同的贡献。由于神经胶质细胞是大脑中的主要细胞类型，我们假设FA和MD的变化意味着神经胶质细胞微观结构的扰动。使用3特斯拉的临床扫描仪，我们进行了体内DTI MRI在9个新生仔猪在20-96小时后，兴奋性脑损伤纹状体喹啉酸注射或全球HI。壳核、尾状核和内囊的FA和MD与星形胶质细胞肿胀、神经元兴奋性毒性和白色物质损伤相关。低FA与水通道蛋白-4（AQP 4）、胶质细胞酸性蛋白（GFAP）和谷氨酸转运蛋白-1（GLT-1）免疫分型的更多肿胀星形胶质细胞相关。低FA还与神经元周围GLT-1+星形胶质细胞装饰的神经元损失、大髓鞘厚度、低髓鞘密度和通过2 '，3'-环核苷酸3 '-磷酸二酯酶、桥接整合子-1和核形态学鉴定的少突胶质细胞死亡相关。MD与退化的少突胶质细胞和正常GFAP+星形胶质细胞的耗竭相关，但与星形胶质细胞或髓鞘肿胀无关。我们得出结论，FA与星形胶质细胞和少突胶质细胞过程中的细胞毒性水肿以及在细胞水平上的髓鞘损伤有关。MD可以检测神经胶质细胞的死亡和损失，但它可能无法辨别肿胀的星形胶质细胞，少突胶质细胞或髓鞘的细微病理。本研究为解释HI后新生儿脑的DTI提供了细胞病理学基础。在新生仔猪兴奋性中毒性脑损伤脑病，部分各向异性扩散张量成像与肿胀的星形胶质细胞免疫表型谷氨酸转运蛋白-1（GLT-1）和正常神经元与神经元周GLT-1+星形胶质细胞装饰在纹状体。
The specific cytopathology that causes abnormal fractional anisotropy (FA) and mean diffusivity (MD) from diffusion tensor imaging (DTI) after neonatal hypoxia-ischemia (HI) is not completely understood. The panoply of cell types in the brain might contribute differentially to changes in DTI metrics. Because glia are the predominant cell type in brain, we hypothesized that changes in FA and MD would signify perturbations in glial microstructure. Using a 3-Tesla clinical scanner, we conducted in vivo DTI MRI in nine neonatal piglets at 20–96 h after excitotoxic brain injury from striatal quinolinic acid injection or global HI. FA and MD from putamen, caudate, and internal capsule in toto were correlated with astrocyte swelling, neuronal excitotoxicity, and white matter injury. Low FA correlated with more swollen astrocytes immunophenotyped by aquaporin-4 (AQP4), glial fibrillary acidic protein (GFAP), and glutamate transporter-1 (GLT-1). Low FA was also related to the loss of neurons with perineuronal GLT-1+ astrocyte decorations, large myelin swellings, lower myelin density, and oligodendrocyte cell death identified by 2’,3’-cyclic nucleotide 3’-phosphodiesterase, bridging integrator-1, and nuclear morphology. MD correlated with degenerating oligodendrocytes and depletion of normal GFAP+ astrocytes but not with astrocyte or myelin swelling. We conclude that FA is associated with cytotoxic edema in astrocytes and oligodendrocyte processes as well as myelin injury at the cellular level. MD can detect glial cell death and loss, but it may not discern subtle pathology in swollen astrocytes, oligodendrocytes, or myelin. This study provides a cytopathologic basis for interpreting DTI in the neonatal brain after HI. In neonatal piglets with encephalopathy from excitotoxic brain injury, fractional anisotropy from diffusion tensor imaging correlates with swollen astrocytes immunophenotyped by glutamate transporter-1 (GLT-1) and normal neurons with perineuronal GLT-1+ astrocyte decorations in the striatum.
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影响因子：
--
作者：
Bylicky MA;Mueller GP;Day RM
通讯作者：
Day RM
DOI：
10.1002/neu.480230915
发表时间：
1992-11-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
CHOI, DW
通讯作者：
CHOI, DW
DOI：
10.1023/b:neur.0000044189.08240.a2
发表时间：
2004-05-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
Hama, K;Arii, T;Ellisman, MH
通讯作者：
Ellisman, MH
影响因子：
2.5
作者：
DOBBING, J;SANDS, J
通讯作者：
SANDS, J
影响因子：
4.4
作者：
Farrell, Jonathan A. D.;Landman, Bennett A.;Mori, Susumu
通讯作者：
Mori, Susumu