Identification and characterization of Pv50, a novel Plasmodium vivax merozoite surface protein
Identification and characterization of Pv50, a novel Plasmodium vivax merozoite surface protein
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Pv50（一种新型间日疟原虫裂殖子表面蛋白）的鉴定和表征
DOI：
10.1186/s13071-019-3434-7
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发表时间：
2019-04
期刊：
影响因子：
--
通讯作者：
Eun-Taek Han
中科院分区：
文献类型：
--
作者：
Yang Cheng;Bo Wang;Feng Lu;Md Atique Ahmed;Jin-Hee Han;Sung Hun Na;Kwon-Soo Ha;Won Sun Park;Seok-Ho Hong;Eun-Taek Han
背景：间日疟原虫含有大约5400个编码基因，其中40%以上编码尚未被功能表征的假设蛋白。在先前使用混合血清样本进行的初步筛选中，从寄生虫分裂期高度转录的蛋白质中选择了许多假设的蛋白质，并确定了包括假设蛋白质在内的高抗原性间日疟原虫候选蛋白。然而，它们在间日疟原虫中的免疫和功能活性尚不清楚。从这些候选物中，我们研究了间日疟原虫50-kDa蛋白（Pv50, pv_087140），该蛋白含有一个高度保守的信号肽，在血期寄生虫中显示出高转录水平。结果：重组Pv50在无细胞表达系统中成功表达，可用于间日疟患者和健康人IgG流行率分析。分析免疫小鼠的免疫应答，并用免疫荧光法检测该蛋白在血期寄生虫体内的亚细胞定位。采用蛋白阵列法评价蛋白-蛋白相互作用，预测寄生虫侵入红细胞过程中蛋白功能的活性。与健康人群相比，重组Pv50在患者样本中显示IgG的流行率，敏感性为42.9%，特异性为93.8%。非嗜细胞性抗体IgG1和IgG3是pv50免疫小鼠抗体应答的主要成分。Pv50定位于分殖子表面，Pv50与PvMSP1之间存在特异性相互作用，提示Pv50-PvMSP1在间日疟原虫中形成异二聚体复合物。结论：检测到本地间日疟原虫引起免疫应答增强，证实了其免疫原性作用。本研究提供了一种检测新的疟疾抗原的方法，Pv50可能是PvMSP1的间日疟疾候选疫苗。
Background:Plasmodium vivax contains approximately 5400 coding genes, more than 40% of which code for hypothetical proteins that have not been functionally characterized. In a previous preliminary screening using pooled serum samples, numerous hypothetical proteins were selected from among those that were highly transcribed in the schizont-stage of parasites, and highly antigenic P. vivax candidates including hypothetical proteins were identified. However, their immunological and functional activities in P. vivax remain unclear. From these candidates, we investigated a P. vivax 50-kDa protein (Pv50, PVX_087140) containing a highly conserved signal peptide that shows high transcription levels in blood-stage parasites.Results:Recombinant Pv50 was expressed in a cell-free expression system and used for IgG prevalence analysis of patients with vivax malaria and healthy individuals. Immune responses were analyzed in immunized mice and mouse antibodies were used to detect the subcellular localization of the protein in blood-stage parasites by immunofluorescence assay. A protein array method was used to evaluate protein-protein interactions to predict protein functional activities during the invasion of parasites into erythrocytes. Recombinant Pv50 showed IgG prevalence in patient samples with a sensitivity of 42.9% and specificity of 93.8% compared to that in healthy individuals. The non-cytophilic antibodies IgG1 and IgG3 were the major components involved in the antibody response in Pv50-immunized mice. Pv50 localized on the surface of merozoites and a specific interaction between Pv50 and PvMSP1 was detected, suggesting that Pv50-PvMSP1 forms a heterodimeric complex in P. vivax.Conclusions:Increased immune responses caused by native P. vivax parasites were detected, confirming its immunogenic effects. This study provides a method for detecting new malaria antigens, and Pv50 may be a vivax malaria vaccine candidate with PvMSP1.
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影响因子：
3.8
作者：
Carlton JM;Sina BJ;Adams JH
通讯作者：
Adams JH
DOI：
10.1073/pnas.95.4.1730
发表时间：
1998-02-17
影响因子：
11.1
作者：
Langhorne, J;Cross, C;von der Weid, T
通讯作者：
von der Weid, T
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
I. Angulo;M. Fresno
通讯作者：
I. Angulo;M. Fresno
影响因子：
3.1
作者：
WHITE, WI;EVANS, CB;TAYLOR, DW
通讯作者：
TAYLOR, DW
DOI：
10.1016/j.bbrc.2008.10.129
发表时间：
2008-12-26
影响因子：
3.1
作者：
Angel, Diana I.;Monguia, Alvaro;Patarroyo, Manuel A.
通讯作者：
Patarroyo, Manuel A.