A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation.
A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation.
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DOI：
10.1016/j.stemcr.2015.04.008
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发表时间：
2015-06-09
影响因子：
5.9
通讯作者：
Shoichet, Molly S.
中科院分区：
文献类型：
--
作者：
Ballios, Brian G.;Cooke, Michael J.;Donaldson, Laura;Coles, Brenda L. K.;Morshead, Cindi M.;van der Kooy, Derek;Shoichet, Molly S.
干细胞及其后代在成人移植模型中的应用一直受到存活率和整合性差的限制。我们设计了一种可注射和生物可吸收的透明质酸和甲基纤维素（HAMC）水凝胶混合物，并在两种细胞类型的两种动物模型中进行了测试，从而了解了它在增强细胞分布、存活、整合和功能修复方面的普遍适用性。HAMC可改善视网膜干细胞（RSC）源性棒的细胞存活和整合。HAMC的促存活机制归因于CD44受体与HA的相互作用。视网膜外限制膜的短暂破坏，结合HAMC的递送，可显著改善视杆存活和视觉功能。HAMC还可以改善神经干细胞和祖细胞（NSCs）的分布、活力和功能修复。HAMC递送系统提高了两种CNS模型的细胞移植效果，表明其具有广泛的适用性。一种可注射的生物材料可改善杆细胞的存活/整合到成人视网膜中，同样的材料可改善神经干细胞在成人大脑中的分布/存活，在视网膜和大脑细胞移植后，功能修复被证明是透明质酸- cd44相互作用对干细胞后代的促存活作用有影响的。Shoicet, van der Kooy，同事们展示了一种可注射的生物材料递送系统如何在动物疾病模型中提高干细胞后代的存活率和功能整合，并将其递送到成人视网膜和大脑。阐明了HAMC对细胞直接促生存作用的机制。
The utility of stem cells and their progeny in adult transplantation models has been limited by poor survival and integration. We designed an injectable and bioresorbable hydrogel blend of hyaluronan and methylcellulose (HAMC) and tested it with two cell types in two animal models, thereby gaining an understanding of its general applicability for enhanced cell distribution, survival, integration, and functional repair relative to conventional cell delivery in saline. HAMC improves cell survival and integration of retinal stem cell (RSC)-derived rods in the retina. The pro-survival mechanism of HAMC is ascribed to the interaction of the CD44 receptor with HA. Transient disruption of the retinal outer limiting membrane, combined with HAMC delivery, results in significantly improved rod survival and visual function. HAMC also improves the distribution, viability, and functional repair of neural stem and progenitor cells (NSCs). The HAMC delivery system improves cell transplantation efficacy in two CNS models, suggesting broad applicability. An injectable biomaterial improves rod survival/integration into adult retina The same material improves neural stem cell distribution/survival into adult brain Functional repair is demonstrated after cell transplantation in both retina and brain Hyaluronan-CD44 interaction is implicated in the pro-survival effect on stem cell progeny Shoicet, van der Kooy, and colleagues demonstrate how an injectable biomaterial delivery system enhances survival and functional integration of stem cell progeny delivered to both adult retina and brain in animal models of disease. The mechanism underlying the direct pro-survival effect of HAMC on cells is elucidated.
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影响因子：
6.3
作者：
Kolb, Bryan;Morshead, Cindi;Weiss, Samuel
通讯作者：
Weiss, Samuel
外部极限膜的药理破坏会导致移植光感受器前体的视网膜整合增加。
影响因子：
3.4
作者：
West, E. L.;Pearson, R. A.;Tschernutter, M.;Sowden, J. C.;MacLaren, R. E.;Ali, R. R.
通讯作者：
Ali, R. R.
影响因子：
64.8
作者：
Pearson, R. A.;Barber, A. C.;Rizzi, M.;Hippert, C.;Xue, T.;West, E. L.;Duran, Y.;Smith, A. J.;Chuang, J. Z.;Azam, S. A.;Luhmann, U. F. O.;Benucci, A.;Sung, C. H.;Bainbridge, J. W.;Carandini, M.;Yau, K. -W.;Sowden, J. C.;Ali, R. R.
通讯作者：
Ali, R. R.
影响因子：
3.3
作者：
Sarthy, V.;Hoshi, H.;Dudley, V. J.
通讯作者：
Dudley, V. J.
影响因子：
16.2
作者：
Rattner, A;Smallwood, PM;Nathans, J
通讯作者：
Nathans, J