Actions of putative embryokines on development of the preimplantation bovine embryo to the blastocyst stage.
Actions of putative embryokines on development of the preimplantation bovine embryo to the blastocyst stage.
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DOI：
10.3168/jds.2020-19068
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发表时间：
2020-12
影响因子：
3.5
通讯作者：
Hansen PJ
中科院分区：
文献类型：
--
作者：
Sang L;Ortiz W;Xiao Y;Estrada-Cortes E;Jannaman EA;Hansen PJ
一旦它在排卵后的第4 - 5天进入子宫，植入前的牛胚胎就被来自母体的称为胚胎因子的调节信号分子控制其发育。在这里，几个细胞信号分子，其基因在子宫内膜中表达在发情后的第5天至第7天进行了测试的能力，影响胚胎的能力，进一步发展和所产生的囊胚的特性。检测的分子为C-利钠肽（CNP）、IL-8、牛形态发生蛋白4（BMP-4）、IL-6和白血病抑制因子（LIF）。测试的细胞信号分子都没有提高胚胎成为囊胚的能力;事实上，BMP-4降低了发育。所有分子都改变了培养物中形成的胚泡的属性。特别是，CNP增加了ICM中的细胞数量，而IL-8减少了内细胞团细胞数量，并倾向于增加孵化或孵化的囊胚比例。此外，BMP-4降低了孵化囊胚的比例。白细胞介素-6和LIF在较小程度上激活了内细胞团中的Janus激酶（JAK）/信号转导子和转录激活子3（STAT 3）信号通路，LIF增加了胚泡中NANOG和磷酸化（激活）STAT 3阳性细胞的百分比。总之，我们的研究结果表明，CNP，IL-8，IL-6，LIF和BMP-4可以修改牛的胚胎发育的方式，影响所得囊胚的特性。需要进一步的研究来了解胚泡特征的这些变化如何影响胚胎建立和维持妊娠的能力。
Once it enters the uterus at d 4 to 5 after ovulation, the preimplantation bovine embryo is controlled in its development by regulatory signaling molecules from the mother called embryokines. Here, several cell-signaling molecules whose genes are expressed in the endometrium during d 5 to 7 after estrus were tested for the ability to affect the competence of the embryo for further development and the characteristics of the resultant blastocysts. Molecules tested were C-natriuretic peptide (CNP), IL-8, bovine morphogenetic protein 4 (BMP-4), IL-6, and leukemia inhibitory factor (LIF). None of the cell-signaling molecules tested improved the competence of the embryo to become a blastocyst; in fact, BMP-4 decreased development. All molecules modified attributes of the blastocyst formed in culture. In particular, CNP increased the number of cells in the ICM, whereas IL-8 decreased inner cell mass cell numbers and tended to increase the proportion of blastocysts that were hatching or hatched. In addition, BMP-4 decreased the proportion of blastocysts that were hatching. Interleukin-6 and, to a lesser extent, LIF activated the Janus kinase (JAK)/signal transducer and activator of transcription 3 (STAT3) signaling pathway in the inner cell mass, and LIF increased the percent of cells in the blastocyst that were positive for both NANOG and phosphorylated (activated) STAT3. In conclusion, our results indicate that CNP, IL-8, IL-6, LIF, and BMP-4 can modify embryonic development of the cow in a manner that affects characteristics of the resultant blastocyst. Further research is required to understand how these changes in characteristics of the blastocyst would affect competence of the embryo to establish and maintain pregnancy.
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DOI：
10.1146/annurev-animal-021419-084010
发表时间：
2020-01-01
期刊：
ANNUAL REVIEW OF ANIMAL BIOSCIENCES, VOL 8, 2020
影响因子：
--
作者：
Hansen，Peter J.
通讯作者：
Hansen，Peter J.
影响因子：
2.8
作者：
Helfrich, Anika L.;Reichenbach, Horst-Dieter;Zerbe, Holm
通讯作者：
Zerbe, Holm
影响因子：
4.8
作者：
Loureiro, Barbara;Bonilla, Luciano;Hansen, Peter J.
通讯作者：
Hansen, Peter J.
影响因子：
3.7
作者：
Boynukalin, Fazilet Kubra;Gultomruk, Meral;Bahceci, Mustafa
通讯作者：
Bahceci, Mustafa
影响因子：
3.8
作者：
Loureiro, Barbara;Block, Jeremy;Hansen, Peter J.
通讯作者：
Hansen, Peter J.