Antibodies from women urogenitally infected with C. trachomatis predominantly recognized the plasmid protein pgp3 in a conformation-dependent manner.
Antibodies from women urogenitally infected with C. trachomatis predominantly recognized the plasmid protein pgp3 in a conformation-dependent manner.
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DOI：
10.1186/1471-2180-8-90
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发表时间：
2008-06-09
期刊：
影响因子：
4.2
通讯作者：
Zhong G
中科院分区：
文献类型：
--
作者：
Li Z;Zhong Y;Lei L;Wu Y;Wang S;Zhong G
沙眼衣原体携带一个隐质粒，编码8个开放阅读框，编号为pORF1至8。目前尚不清楚是否所有8个porf在人类沙眼衣原体感染期间表达，关于质粒蛋白功能的信息也非常有限。当来自泌尿生殖道感染沙眼衣原体妇女的抗体与质粒蛋白反应时，所有8种porf均被一种或多种人抗体样品阳性识别，其中大多数抗体识别pORF5蛋白（称为pgp3），且滴度最高。沙眼衣原体感染的HeLa能阻断porf的抗体识别，而正常的HeLa细胞裂解物则不能。pgp3融合蛋白纯化的人IgG在沙眼衣原体感染细胞的胞浆中检测到内源性pgp3，其胞内分布模式与衣原体基因组编码蛋白酶因子CPAF相似。然而，当两种抗原线性化或热变性时，人抗体不再识别pgp3，但仍能识别CPAF。pgp3的构象可能是由c端75%的氨基酸序列维持的，因为进一步的删除阻断了人抗体和两种构象依赖的小鼠单克隆抗体的结合。质粒编码的8种蛋白在人衣原体感染过程中均有表达和免疫原性，其中pgp3是最具免疫优势的抗原。更重要的是，人类抗pgp3抗体是高度构象依赖的。这些观察结果为进一步了解质粒编码蛋白的功能和探索pgp3在衣原体诊断和疫苗接种中的应用提供了重要信息。
C. trachomatis organisms carry a cryptic plasmid that encodes 8 open reading frames designated as pORF1 to 8. It is not clear whether all 8 pORFs are expressed during C. trachomatis infection in humans and information on the functionality of the plasmid proteins is also very limited. When antibodies from women urogenitally infected with C. trachomatis were reacted with the plasmid proteins, all 8 pORFs were positively recognized by one or more human antibody samples with the recognition of pORF5 protein (known as pgp3) by most antibodies and with the highest titers. The antibody recognition of the pORFs was blocked by C. trachomatis-infected HeLa but not normal HeLa cell lysates. The pgp3 fusion protein-purified human IgG detected the endogenous pgp3 in the cytosol of C. trachomatis-infected cells with an intracellular distribution pattern similar to that of CPAF, a chlamydial genome-encoded protease factor. However, the human antibodies no longer recognized pgp3 but maintained recognition of CPAF when both antigens were linearized or heat-denatured. The pgp3 conformation is likely maintained by the C-terminal 75% amino acid sequence since further deletion blocked the binding by the human antibodies and two conformation-dependent mouse monoclonal antibodies. The plasmid-encoded 8 proteins are both expressed and immunogenic with pgp3 as the most immunodominant antigen during chlamydial infection in humans. More importantly, the human anti-pgp3 antibodies are highly conformation-dependent. These observations have provided important information for further understanding the function of the plasmid-encoded proteins and exploring the utility of pgp3 in chlamydial diagnosis and vaccination.
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影响因子：
4.6
作者：
Ghaem-Maghami, S;Ratti, G;Lewis, DJM
通讯作者：
Lewis, DJM
影响因子：
3.1
作者：
Murthy, Ashlesh K.;Chambers, James P.;Arulanandam, Bernard P.
通讯作者：
Arulanandam, Bernard P.
影响因子：
5.5
作者：
Bas, S;Genevay, S;Vischer, TL
通讯作者：
Vischer, TL
影响因子：
3.1
作者：
COMANDUCCI, M;MANETTI, R;RATTI, G
通讯作者：
RATTI, G
DOI：
10.1099/00221287-139-5-1083
发表时间：
1993-05-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
COMANDUCCI, M;CEVENINI, R;RATTI, G
通讯作者：
RATTI, G