Bio-inspired dual-functional phospholipid-poly(acrylic acid) brushes grafted porous poly(vinyl alcohol) beads for selective adsorption of low-density lipoprotein.
Bio-inspired dual-functional phospholipid-poly(acrylic acid) brushes grafted porous poly(vinyl alcohol) beads for selective adsorption of low-density lipoprotein.
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仿生双功能磷脂-聚丙烯酸刷接枝多孔聚乙烯醇珠，用于选择性吸附低密度脂蛋白。
DOI：
10.1039/d1tb01220g
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发表时间：
2021-07
期刊：
影响因子：
--
通讯作者：
Yamin Chai
中科院分区：
文献类型：
--
作者：
Lisha Sun;Zhuang Liu;Lichun Wang;Jingzhe Dong;Yameng Yu;Boya Ma;Xinbang Jiang;Lailiang Ou;Wenzhong Li;Chen Guo;Yamin Chai
低密度脂蛋白（LDL）水平升高被认为是高脂血症（HLP）的重要指标，降低LDL水平代表了有效的临床治疗策略。受磷脂单分子层和LDL颗粒的脂质含量的缀合的启发，目前的研究描述了一种创新的血液灌流吸附剂的制备。通过将磷脂酰乙醇胺附着到聚（丙烯酸）改性的聚（乙烯醇-co-三烯丙基异氰脲酸酯）珠（PVA@PAA-PE）上制备吸附剂。LDL和吸附剂之间的相互作用模拟了血液中存在的脂蛋白微乳液，从而促进了高亲和力的有效结合。体外吸附实验表明，PVA@ PAA-PE对LDL的吸附量是对照组的4.44倍，对LDL的去除率约为抗动脉粥样硬化高密度脂蛋白（HDL）的2倍。体内全血灌注证明PVA@PAA-PE对LDL具有上级亲和力，因为LDL浓度从10.71 ± 2.36 mmol L-1显著降低至6.21 ± 1.45 mmol L-1，而HDL水平没有严重降低（从0.98 ± 0.12 mmol L-1降低至0.56 ± 0.15 mmol L-1）。此外，PVA@PAA-PE具有优异的血液相容性和低细胞毒性。因此，PVA@PAA-PE是一种潜在的全血灌流治疗高脂血症的吸附剂。
Elevated levels of low-density lipoproteins (LDL) are recognized as a crucial indicator of hyperlipidemia (HLP) and lowering of LDL levels represents an effective clinical treatment strategy. Inspired by the conjugation of phospholipid monolayers and the lipid content of the LDL particle, the current study describes the preparation of an innovative hemoperfusion adsorbent. The adsorbent was prepared by attachment of phosphatidyl ethanolamine to poly(acrylic acid) modified poly(vinyl alcohol-co-triallyl isocyanurate) beads (PVA@PAA-PE). The interaction between LDL and adsorbent mimics the lipoprotein microemulsion present in the blood and thus promotes efficient binding with high affinity. In vitro adsorption using serum from patients with HLP revealed that the LDL adsorption of PVA@PAA-PE was 4.44 times higher than that of controls and the removal rate of LDL using PVA@PAA-PE was about twice as high as that of the anti-atherogenic high-density lipoprotein (HDL). In vivo whole blood perfusion demonstrated the superior affinity of PVA@PAA-PE for LDL since LDL concentration was significantly reduced from 10.71 ± 2.36 mmol L-1 to 6.21 ± 1.45 mmol L-1, while the HDL level was not severely reduced (from 0.98 ± 0.12 mmol L-1 to 0.56 ± 0.15 mmol L-1). Additionally, PVA@PAA-PE exhibited excellent hemocompatibility and low cytotoxicity. Therefore, PVA@PAA-PE is a potential adsorbent for whole blood perfusion to treat hyperlipidemia.
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影响因子：
14
作者：
Yan Cheng;Shenqi Wang;Yao-ting Yu;Yi Yuan
通讯作者：
Yan Cheng;Shenqi Wang;Yao-ting Yu;Yi Yuan
影响因子：
9.5
作者：
Fanrou Zhang;Qing-xin Song;Xuan Huang;Feng-ning Li;Kun Wang;Yi-xing Tang;Cang-long Hou;Hongxing Shen
通讯作者：
Fanrou Zhang;Qing-xin Song;Xuan Huang;Feng-ning Li;Kun Wang;Yi-xing Tang;Cang-long Hou;Hongxing Shen
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影响因子：
14
作者：
Chen, Hao;Sun, Tao;Zhang, Hongyu
通讯作者：
Zhang, Hongyu
DOI：
10.1002/jcph.866
发表时间：
2017-07
期刊：
The Journal of Clinical Pharmacology
影响因子：
--
作者：
N. Scherer;Christiane Dings;M. Böhm;U. Laufs;T. Lehr
通讯作者：
N. Scherer;Christiane Dings;M. Böhm;U. Laufs;T. Lehr
影响因子：
--
作者：
Lund-Katz, Sissel;Phillips, Michael C.
通讯作者：
Phillips, Michael C.